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  4. Janelia Fluor® 549, Azide

Janelia Fluor® 549, Azide  (Synonyms: JF549, Azide)

Cat. No.: HY-131021
SDS
Handling Instructions Technical Support

Janelia Fluor? 549, Azide (JF549, Azide) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

Janelia Fluor® 549, Azide Chemical Structure

Janelia Fluor® 549, Azide Chemical Structure

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5 mg In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Janelia Fluor® 549, Azide:

Janelia Fluor® 549, Azide Related Antibodies

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Description

Janelia Fluor? 549, Azide (JF549, Azide) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1]. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

Molecular Weight

654.71

Formula

C35H38N6O7
Appearance

Oil

Color

Dark purple to black

Emission (Em)

549
Excitation (Ex)

505
SMILES

O=C(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)NCCOCCOCCOCCN=[N+]=[N-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (76.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5274 mL 7.6370 mL 15.2739 mL
5 mM 0.3055 mL 1.5274 mL 3.0548 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5274 mL 7.6370 mL 15.2739 mL 38.1848 mL
5 mM 0.3055 mL 1.5274 mL 3.0548 mL 7.6370 mL
10 mM 0.1527 mL 0.7637 mL 1.5274 mL 3.8185 mL
15 mM 0.1018 mL 0.5091 mL 1.0183 mL 2.5457 mL
20 mM 0.0764 mL 0.3818 mL 0.7637 mL 1.9092 mL
25 mM 0.0611 mL 0.3055 mL 0.6110 mL 1.5274 mL
30 mM 0.0509 mL 0.2546 mL 0.5091 mL 1.2728 mL
40 mM 0.0382 mL 0.1909 mL 0.3818 mL 0.9546 mL
50 mM 0.0305 mL 0.1527 mL 0.3055 mL 0.7637 mL
60 mM 0.0255 mL 0.1273 mL 0.2546 mL 0.6364 mL
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Janelia Fluor® 549, Azide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 549, AzideJF549, AzideFluorescent DyeInhibitorinhibitorinhibit

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