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  4. Janelia Fluor® 549 TFA

Janelia Fluor® 549 TFA  (Synonyms: JF549 TFA)

Cat. No.: HY-131022 Purity: 96.56%
SDS COA Handling Instructions

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Janelia Fluor® 549 TFA (JF549 TFA) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 549 TFA Chemical Structure

Janelia Fluor® 549 TFA Chemical Structure

CAS No. : 2245946-45-4

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5 mg USD 430 In-stock
10 mg USD 730 In-stock
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This product is a controlled substance and not for sale in your territory.

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Description

Janelia Fluor® 549 TFA (JF549 TFA) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

Molecular Weight

568.50

Formula

C29H23F3N2O7
CAS No.
Appearance

Solid

Color

Light brown to brown

Emission (Em)

549
Excitation (Ex)

505
SMILES

O=C(C1=CC=C(C(O)=O)C(C2=C3C=CC(N4CCC4)=CC3=[O+]C5=C2C=CC(N6CCC6)=C5)=C1)[O-].O=C(O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (43.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7590 mL 8.7951 mL 17.5902 mL
5 mM 0.3518 mL 1.7590 mL 3.5180 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.56%

SDS (252 KB)

COA (251 KB) RP-HPLC (139 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7590 mL 8.7951 mL 17.5901 mL 43.9754 mL
5 mM 0.3518 mL 1.7590 mL 3.5180 mL 8.7951 mL
10 mM 0.1759 mL 0.8795 mL 1.7590 mL 4.3975 mL
15 mM 0.1173 mL 0.5863 mL 1.1727 mL 2.9317 mL
20 mM 0.0880 mL 0.4398 mL 0.8795 mL 2.1988 mL
25 mM 0.0704 mL 0.3518 mL 0.7036 mL 1.7590 mL
30 mM 0.0586 mL 0.2932 mL 0.5863 mL 1.4658 mL
40 mM 0.0440 mL 0.2199 mL 0.4398 mL 1.0994 mL
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Janelia Fluor® 549 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 5492245946-45-4JF549JF 549JF-549Fluorescent DyeInhibitorinhibitorinhibit

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