L-Asparaginase  (Synonyms: L-​ASNase)

L-Asparaginase (L-ASNase) is a deamidating enzyme that catalyses the hydrolysis of L-asparagine and L-glutamine, and can be used for the research of acute lymphoblastic leukemia. L-Asparaginase depletes L-asparagine from plasma resulting in inhibition of RNA and DNA synthesis with the subsequent blastic cell apoptosis^[1].

L-Asparaginase has a cytotoxic effect on AML blasts and LSC subpopulations^[1].
L-Asparaginase is effective on both CD34⁺CD38⁺ and CD34⁺CD38⁺ LSC fractions, MSC and monocytes/macrophages partially counteracted the effect^[1].
Although lyophilized L-asparaginase is stable at room temperature for 3 weeks, it should be stored dry below -18°C. L-Asparaginase should Co-culture with cells at 37°C, 5% CO2 for 24, 48 h.
L-Asparaginase (100 IU/mL, 0-24 h) is co-cultured with L5178Y and B16 cells (5×10⁵/mL), and cells are arrested in the G1 phase and DNA strand breaks occurred^[2].
Product Information
Optimal pH: 8.0-9.0
Agonists: Anaerobic conditions, amino acids
Inhibitors: 5-diazo-4-o-L-norvaline (DONV), dimethyl sulfoxide, glutaraldehyde, L-asparagine and L-aspartic acid, NH₄⁺, trypsin

Instructions
Dissolve in 1 mg/ml 0.2 M Tris-HCl pH 8.6, store at 2-8℃ after dissolution, and use as soon as possible within 1 week. Or dissolve according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

34000 (monomer)

9015-68-3

Solid

White to off-white

[L-Asparaginase]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ilaria M Michelozzi, et al. Acute myeloid leukaemia niche regulates response to L-asparaginase. Br J Haematol. 2019 Aug;186(3):420-430. doi: 10.1111/bjh.15920.  [Content Brief]

  [2]. T Ueno, et al. Cell cycle arrest and apoptosis of leukemia cells induced by L-asparaginase. Leukemia. 1997 Nov;11(11):1858-61.  [Content Brief]