L-Histidine benzyl ester bistosylate

Name: L-Histidine benzyl ester bistosylate
Brand: MedChemExpress
SKU: HY-138652
Rating: 4.5 (1 reviews)

Cat. No.: HY-138652 Purity: 99.40%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

L-Histidine benzyl ester bistosylate could play a role in the activation of HutP (an RNA-binding protein).

For research use only. We do not sell to patients.

L-Histidine benzyl ester bistosylate Chemical Structure

CAS No. : 24593-59-7

Size	Price	Stock	Quantity
1 g	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g	USD 150	In-stock	Estimated Time of Arrival: December 31
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50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

L-Histidine benzyl ester bistosylate could play a role in the activation of HutP (an RNA-binding protein)^[1].

Molecular Weight

589.68

Formula

C₂₇H₃₁N₃O₈S₂

CAS No.

24593-59-7

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CC1=CNC=N1)C(OCC2=CC=CC=C2)=O.O=S(C3=CC=C(C)C=C3)(O)=O.O=S(C4=CC=C(C)C=C4)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (423.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (169.58 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6958 mL	8.4792 mL	16.9583 mL
5 mM	0.3392 mL	1.6958 mL	3.3917 mL
10 mM	0.1696 mL	0.8479 mL	1.6958 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (277 KB) SDS (392 KB)

COA (256 KB) HNMR (289 KB) RP-HPLC (209 KB) MS (172 KB)

Handling Instructions (2659 KB)

References

[1]. Thirumananseri Kumarevel, et al. Allosteric activation of HutP protein, that regulates transcription of hut operon in Bacillus subtilis, mediated by various analogs of histidine. Nucleic Acids Res Suppl. 2003;(3):199-200. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.6958 mL	8.4792 mL	16.9584 mL	42.3959 mL
	5 mM	0.3392 mL	1.6958 mL	3.3917 mL	8.4792 mL
	10 mM	0.1696 mL	0.8479 mL	1.6958 mL	4.2396 mL
	15 mM	0.1131 mL	0.5653 mL	1.1306 mL	2.8264 mL
	20 mM	0.0848 mL	0.4240 mL	0.8479 mL	2.1198 mL
	25 mM	0.0678 mL	0.3392 mL	0.6783 mL	1.6958 mL
	30 mM	0.0565 mL	0.2826 mL	0.5653 mL	1.4132 mL
	40 mM	0.0424 mL	0.2120 mL	0.4240 mL	1.0599 mL
	50 mM	0.0339 mL	0.1696 mL	0.3392 mL	0.8479 mL
	60 mM	0.0283 mL	0.1413 mL	0.2826 mL	0.7066 mL
	80 mM	0.0212 mL	0.1060 mL	0.2120 mL	0.5299 mL
	100 mM	0.0170 mL	0.0848 mL	0.1696 mL	0.4240 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.