2. Others
  3. Fluorescent Dye
  4. L-Leucine-7-amido-4-methylcoumarin hydrochloride

L-Leucine-7-amido-4-methylcoumarin hydrochloride  (Synonyms: Leu-AMC hydrochloride)

Cat. No.: HY-137844 Purity: 99.17%
COA Handling Instructions

Molarity Calculator

L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro.

For research use only. We do not sell to patients.

L-Leucine-7-amido-4-methylcoumarin hydrochloride Chemical Structure

L-Leucine-7-amido-4-methylcoumarin hydrochloride Chemical Structure

CAS No. : 62480-44-8

Size Price Stock Quantity
5 mg USD 90 In-stock
10 mg USD 140 In-stock
25 mg USD 290 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of L-Leucine-7-amido-4-methylcoumarin hydrochloride:

L-Leucine-7-amido-4-methylcoumarin hydrochloride Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro[1].

In Vitro

In leucine aminopeptidases inhibition assays in KB 3.1 cells. Compounds3and5are observed to weakly inhibit the biofilm formation byS. aureusand constrain inhibitory activity of -Leu-AMC hydrolysis in KB 3.1 cells[1].
The amount of hydrolyzed 7-amino-4-methylcoumarin (AMC) is determined in a fluorescence spectrophotometer (Ex/Em=365/440 nm)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Leucine-7-amido-4-methylcoumarin hydrochloride Related Antibodies
Molecular Weight

324.80

Formula

C16H21ClN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C(C(O1)=C2)=CC=C2NC([C@@H](N)CC(C)C)=O)=CC1=O.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (307.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0788 mL 15.3941 mL 30.7882 mL
5 mM 0.6158 mL 3.0788 mL 6.1576 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0788 mL 15.3941 mL 30.7882 mL 76.9704 mL
5 mM 0.6158 mL 3.0788 mL 6.1576 mL 15.3941 mL
10 mM 0.3079 mL 1.5394 mL 3.0788 mL 7.6970 mL
15 mM 0.2053 mL 1.0263 mL 2.0525 mL 5.1314 mL
20 mM 0.1539 mL 0.7697 mL 1.5394 mL 3.8485 mL
25 mM 0.1232 mL 0.6158 mL 1.2315 mL 3.0788 mL
30 mM 0.1026 mL 0.5131 mL 1.0263 mL 2.5657 mL
40 mM 0.0770 mL 0.3849 mL 0.7697 mL 1.9243 mL
50 mM 0.0616 mL 0.3079 mL 0.6158 mL 1.5394 mL
60 mM 0.0513 mL 0.2566 mL 0.5131 mL 1.2828 mL
80 mM 0.0385 mL 0.1924 mL 0.3849 mL 0.9621 mL
100 mM 0.0308 mL 0.1539 mL 0.3079 mL 0.7697 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

L-Leucine-7-amido-4-methylcoumarin hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Leucine-7-amido-4-methylcoumarin62480-44-8Leu-AMCFluorescent Dyefluorogenic peptidyl substrateLAP3leucine aminopeptidaseAMCKB 3.1cellInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
L-Leucine-7-amido-4-methylcoumarin hydrochloride
Cat. No.:
HY-137844
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.