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  2. Biochemical Assay Reagents
  3. L-Leucyl-4-nitroanilide hydrochloride

L-Leucyl-4-nitroanilide hydrochloride is a commonly used substrate in various biochemical assays to measure the activity of enzymes that cleave peptide bonds, such as proteases. L-Leucine p-nitroanilide hydrochloride has unique chemical properties that allow it to be cleaved by these enzymes to form a yellow product called p-nitroaniline. This makes it a useful tool for detecting and quantifying protease activity in biological samples such as blood or tissue extracts.

For research use only. We do not sell to patients.

L-Leucyl-4-nitroanilide hydrochloride Chemical Structure

L-Leucyl-4-nitroanilide hydrochloride Chemical Structure

CAS No. : 16010-98-3

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Description

L-Leucyl-4-nitroanilide hydrochloride is a commonly used substrate in various biochemical assays to measure the activity of enzymes that cleave peptide bonds, such as proteases. L-Leucine p-nitroanilide hydrochloride has unique chemical properties that allow it to be cleaved by these enzymes to form a yellow product called p-nitroaniline. This makes it a useful tool for detecting and quantifying protease activity in biological samples such as blood or tissue extracts.

In Vitro

L-Leucine p-nitroanilide (hydrochloride) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

287.74

Formula

C12H18ClN3O3

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

N[C@@H](CC(C)C)C(NC1=CC=C([N+]([O-])=O)C=C1)=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation

Purity: 99.97%

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L-Leucyl-4-nitroanilide hydrochloride Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
L-Leucyl-4-nitroanilide hydrochloride
Cat. No.:
HY-137872
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