LH1307

Cat. No.: HY-132212 Purity: 96.9%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

LH1307 is a C2-symmetric inhibitor of PD-1/PD-L1 protein-protein interactions with a IC₅₀ value of 3.0 μM and can be used in cancer studies.

For research use only. We do not sell to patients.

LH1307 Chemical Structure

CAS No. : 2375720-38-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Description

LH1307 is a C2-symmetric inhibitor of PD-1/PD-L1 protein-protein interactions with a IC₅₀ value of 3.0 μM and can be used in cancer studies^[1].

IC₅₀ & Target

IC50: 3.0 μM (PD-1/PD-L1)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-K1	GI₅₀	11.8 μM Compound: 2b	Cytotoxicity against CHOK1 cells stably expressing human PDL1 co-cultured with human Jurkat T cell expressing human PD1 assessed as growth inhibition measured after 24 hrs by XTT assay Cytotoxicity against CHOK1 cells stably expressing human PDL1 co-cultured with human Jurkat T cell expressing human PD1 assessed as growth inhibition measured after 24 hrs by XTT assay	[PMID: 31298541]
HEK293	IC₅₀	3 nM Compound: 2b	Inhibition of human Fc-tagged PD1 N-terminal domain (Leu25 to Gln167 residues) expressed in HEK293 cells/human His-tagged PDL1 (Phe19 to Arg238 residues) expressed in HEK293 cells protein-protein interaction after 1 hr by APC-labeled anti-His antibody/Eu- Inhibition of human Fc-tagged PD1 N-terminal domain (Leu25 to Gln167 residues) expressed in HEK293 cells/human His-tagged PDL1 (Phe19 to Arg238 residues) expressed in HEK293 cells protein-protein interaction after 1 hr by APC-labeled anti-His antibody/Eu-	[PMID: 31298541]
Jurkat	GI₅₀	11.8 μM Compound: 2b	Cytotoxicity against human Jurkat T cell expressing human PD1 co-cultured with CHOK1 cells stably expressing human PDL1 assessed as growth inhibition measured after 24 hrs by XTT assay Cytotoxicity against human Jurkat T cell expressing human PD1 co-cultured with CHOK1 cells stably expressing human PDL1 assessed as growth inhibition measured after 24 hrs by XTT assay	[PMID: 31298541]
Jurkat	EC₅₀	763 nM Compound: 2b	Inhibition of human PD1 stably expressed in human Jurkat T cells co-expressing NFAT-induced luciferase/human PDL1 stably expressed in CHOK1 cells co-expressing aAPC protein-protein interaction assessed as increase in NFAT signaling measured after 6 hrs by Inhibition of human PD1 stably expressed in human Jurkat T cells co-expressing NFAT-induced luciferase/human PDL1 stably expressed in CHOK1 cells co-expressing aAPC protein-protein interaction assessed as increase in NFAT signaling measured after 6 hrs by	[PMID: 31298541]
Jurkat	EC₅₀	79 nM Compound: 2b	Inhibition of PD1/PDL1 interaction in human Jurkat cells co-cultured with human U2OS cells expressing PDL1 assessed as reduction in SHP1 recruitment preincubated with U2OS cells for 60 mins followed by Jurkat cell addition and measured after 2 hrs by Path Inhibition of PD1/PDL1 interaction in human Jurkat cells co-cultured with human U2OS cells expressing PDL1 assessed as reduction in SHP1 recruitment preincubated with U2OS cells for 60 mins followed by Jurkat cell addition and measured after 2 hrs by Path	[PMID: 31298541]

Molecular Weight

915.09

Formula

C₅₄H₅₈N₈O₆

CAS No.

2375720-38-8

Appearance

Solid

Color

Off-white to light yellow

SMILES

N#CC1=CN=CC(COC2=CC(OCC3=CC=CC(C4=CC=CC(COC5=C(C)C=C(CNCCNC(C)=O)C(OCC6=CC(C#N)=CN=C6)=C5)=C4C)=C3C)=C(C)C=C2CNCCNC(C)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Purity & Documentation

Purity: 96.9%

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Data Sheet (264 KB) SDS (251 KB)

COA (245 KB)

Handling Instructions (2659 KB)

References

[1]. Basu S, et al. Design, Synthesis, Evaluation, and Structural Studies of C2-Symmetric Small Molecule Inhibitors of Programmed Cell Death-1/Programmed Death-Ligand 1 Protein-Protein Interaction. J Med Chem. 2019 Aug 8;62(15):7250-7263. [Content Brief]

LH1307 Related Classifications

Molarity Calculator
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LH13072375720-38-8LH 1307LH-1307PD-1/PD-L1PD-1/Programmed death-ligand 1C2-symmetric inhibitorPD-1/PD-L1 protein-protein interactionInhibitorinhibitorinhibit

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