2. Cell Cycle/DNA Damage TGF-beta/Smad Stem Cell/Wnt Cytoskeleton
  3. LIM Kinase (LIMK) ROCK
  4. LIMK-IN-1

LIMK-IN-1 (Compound 14) is an inhibitor of LIM-Kinase (LIMK), with IC50s of 0.5 nM and 0.9 nM for LIMK1 and LIMK2, respectively. LIMK-IN-1 can be used for ocular hypertension and associated glaucoma research.

For research use only. We do not sell to patients.

LIMK-IN-1 Chemical Structure

LIMK-IN-1 Chemical Structure

CAS No. : 1116571-01-7

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Based on 1 publication(s) in Google Scholar

LIMK-IN-1 Related Antibodies

Top Publications Citing Use of Products

View All LIM Kinase (LIMK) Isoform Specific Products:

View All Isoforms
LIMK1 LIMK2

View All ROCK Isoform Specific Products:

View All Isoforms
ROCK ROCK1 ROCK2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

LIMK-IN-1 (Compound 14) is an inhibitor of LIM-Kinase (LIMK), with IC50s of 0.5 nM and 0.9 nM for LIMK1 and LIMK2, respectively. LIMK-IN-1 can be used for ocular hypertension and associated glaucoma research[1].

IC50 & Target[1]

LIMK1

0.9 nM (IC50)

LIMK2

0.5 nM (IC50)

ROCK1

260 nM (IC50)

ROCK2

2700 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
0.9 nM
Compound: 14
Inhibition of human recombinant LIMK2 expressed in baculovirus-Sf9 system by scintillation counting
Inhibition of human recombinant LIMK2 expressed in baculovirus-Sf9 system by scintillation counting
[PMID: 19831390]
In Vitro

LIMK-IN-1 (Compound 14) (100 nM) increases outflow facility by about 30% in a pig eye perfusion assay[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LIMK-IN-1 Related Antibodies
Molecular Weight

453.34

Formula

C20H21BrN8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=CNC2=NC=NC(N3C[C@H](C)N(/C(NC4=CC=CC(Br)=C4)=N\C#N)CC3)=C21
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (275.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2058 mL 11.0293 mL 22.0585 mL
5 mM 0.4412 mL 2.2058 mL 4.4117 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2058 mL 11.0292 mL 22.0585 mL 55.1462 mL
5 mM 0.4412 mL 2.2058 mL 4.4117 mL 11.0292 mL
10 mM 0.2206 mL 1.1029 mL 2.2058 mL 5.5146 mL
15 mM 0.1471 mL 0.7353 mL 1.4706 mL 3.6764 mL
20 mM 0.1103 mL 0.5515 mL 1.1029 mL 2.7573 mL
25 mM 0.0882 mL 0.4412 mL 0.8823 mL 2.2058 mL
30 mM 0.0735 mL 0.3676 mL 0.7353 mL 1.8382 mL
40 mM 0.0551 mL 0.2757 mL 0.5515 mL 1.3787 mL
50 mM 0.0441 mL 0.2206 mL 0.4412 mL 1.1029 mL
60 mM 0.0368 mL 0.1838 mL 0.3676 mL 0.9191 mL
80 mM 0.0276 mL 0.1379 mL 0.2757 mL 0.6893 mL
100 mM 0.0221 mL 0.1103 mL 0.2206 mL 0.5515 mL
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LIMK-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LIMK-IN-11116571-01-7LIM Kinase (LIMK)ROCKLIMKsRho-associated protein kinaseRho-associated kinaseRho-kinaseROKLIM-Kinase 2 InhibitorsOcular HypertensionAssociated GlaucomaInhibitorinhibitorinhibit

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