Mal-amido-PEG2-TFP ester

Name: Mal-amido-PEG2-TFP ester
Brand: MedChemExpress
SKU: HY-130232
Rating: 4.5 (1 reviews)

Cat. No.: HY-130232 Purity: 95.09%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Mal-amido-PEG2-TFP ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

Mal-amido-PEG2-TFP ester Chemical Structure

CAS No. : 1431291-44-9

Size	Price	Stock	Quantity
50 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 99	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 164	Get quote	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All PROTAC Linkers Isoform Specific Products:

View All Isoforms

PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers

Biological Activity
Purity & Documentation
References
Customer Review

Description

Mal-amido-PEG2-TFP ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs^[1].

IC₅₀ & Target

PEGs	Alkyl/ether

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

476.38

Formula

C₂₀H₂₀F₄N₂O₇

CAS No.

1431291-44-9

Appearance

Solid

SMILES

O=C(OC1=C(F)C(F)=CC(F)=C1F)CCOCCOCCNC(CCN2C(C=CC2=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 150 mg/mL (314.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.71 mg/mL (5.69 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0992 mL	10.4958 mL	20.9916 mL
5 mM	0.4198 mL	2.0992 mL	4.1983 mL
10 mM	0.2099 mL	1.0496 mL	2.0992 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3.75 mg/mL (7.87 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.75 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.75 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.09%

Select Batch:

Data Sheet (269 KB) SDS (251 KB)

COA (244 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0992 mL	10.4958 mL	20.9916 mL	52.4791 mL
H₂O / DMSO	5 mM	0.4198 mL	2.0992 mL	4.1983 mL	10.4958 mL
DMSO	10 mM	0.2099 mL	1.0496 mL	2.0992 mL	5.2479 mL
	15 mM	0.1399 mL	0.6997 mL	1.3994 mL	3.4986 mL
	20 mM	0.1050 mL	0.5248 mL	1.0496 mL	2.6240 mL
	25 mM	0.0840 mL	0.4198 mL	0.8397 mL	2.0992 mL
	30 mM	0.0700 mL	0.3499 mL	0.6997 mL	1.7493 mL
	40 mM	0.0525 mL	0.2624 mL	0.5248 mL	1.3120 mL
	50 mM	0.0420 mL	0.2099 mL	0.4198 mL	1.0496 mL
	60 mM	0.0350 mL	0.1749 mL	0.3499 mL	0.8747 mL
	80 mM	0.0262 mL	0.1312 mL	0.2624 mL	0.6560 mL
	100 mM	0.0210 mL	0.1050 mL	0.2099 mL	0.5248 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.