MARK4 inhibitor 1

Name: MARK4 inhibitor 1
Brand: MedChemExpress
SKU: HY-114317
Rating: 4.5 (1 reviews)

Cat. No.: HY-114317 Purity: 98.29%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

MARK4 inhibitor 1 is a potent and selective microtubule affinity-regulating kinase 4 (MARK4) inhibitor, with an IC₅₀ of 1.54 μM. MARK4 inhibitor 1 inhibits cancer cell proliferation, metastasis and induces apoptosis.

For research use only. We do not sell to patients.

MARK4 inhibitor 1 Chemical Structure

CAS No. : 2271081-58-2

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 1.54 μM (MARK4)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	200 μM Compound: 9g	Antiproliferative activity against human HEK293 cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human HEK293 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 30579124]
HepG2	IC₅₀	8.14 μM Compound: 9g	Antiproliferative activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 30579124]
MCF7	IC₅₀	6.22 μM Compound: 9g	Antiproliferative activity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 30579124]
MDA-MB-435S	IC₅₀	9.94 μM Compound: 9g	Antiproliferative activity against human MDA-MB-435S cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-435S cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 30579124]

In Vitro

MARK4 inhibitor 1 (compound 9g; 0-200 µM ; 24-48 h) shows inhibition of cell proliferation and cell migration^[1].
MARK4 inhibitor 1 (compound 9g; 24) induces apoptosis in these cancerous cells, with IC₅₀ values of 6.22 μM, 9.94 μM and 8.14 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	MCF-7, MDA-MB-435s and HepG2 cells
Concentration:	0-200 µM
Incubation Time:	24 and 48 h
Result:	Decreased the viability of these cells in a concentration dependent manner.

Molecular Weight

390.40

Formula

C₂₀H₁₈N₆O₃

CAS No.

2271081-58-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CN(CC2=CC=C(OC)C=C2)N=N1)N/N=C3C(NC4=C/3C=CC=C4C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (12.81 mM; ultrasonic and adjust pH to 2 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5615 mL	12.8074 mL	25.6148 mL
5 mM	0.5123 mL	2.5615 mL	5.1230 mL
10 mM	0.2561 mL	1.2807 mL	2.5615 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.29%

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Data Sheet (275 KB) SDS (252 KB)

COA (252 KB) HNMR (217 KB) LCMS (134 KB)

Handling Instructions (2659 KB)

References

[1]. Aneja B, et al. Design and development of Isatin-triazole hydrazones as potential inhibitors of microtubule affinity-regulating kinase 4 for the therapeutic management of cell proliferation and metastasis. Eur J Med Chem. 2019 Feb 1;163:840-852. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5615 mL	12.8074 mL	25.6148 mL	64.0369 mL
	5 mM	0.5123 mL	2.5615 mL	5.1230 mL	12.8074 mL
	10 mM	0.2561 mL	1.2807 mL	2.5615 mL	6.4037 mL