MBD (Synonyms: 7-(p-Methoxybenzylamino)-4-nitrobenz-2,1,3-oxadiazole)

Cat. No.: HY-141667 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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MBD (7-(p-Methoxybenzylamino)-4-nitrobenz-2,1,3-oxadiazole), a new fluorescent probe for protein and nucleoprotein conformation, is applied to bacterial ribosomes and to bovine trypsinogen and trypsin. MBD is strongly fluorescent upon binding to a hydrophobic area of a macromolecule.

For research use only. We do not sell to patients.

MBD Chemical Structure

CAS No. : 33984-50-8

Size	Price	Stock	Quantity
50 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Description

Molecular Weight

300.27

Formula

C₁₄H₁₂N₄O₄

CAS No.

33984-50-8

Appearance

Solid

Color

Orange to red

SMILES

O=[N+](C1=CC=C(NCC2=CC=C(OC)C=C2)C3=NON=C31)[O-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (83.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3303 mL	16.6517 mL	33.3034 mL
5 mM	0.6661 mL	3.3303 mL	6.6607 mL
10 mM	0.3330 mL	1.6652 mL	3.3303 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (248 KB)

Handling Instructions (2659 KB)

References

[1]. Kenner RA, et al. A new fluorescent probe for protein and nucleoprotein conformation. Binding of 7-(p-methoxybenzylamino)-4-nitrobenzoxadiazole to bovine trypsinogen and bacterial ribosomes. Biochemistry. 1971;10(24):4433-4440. [Content Brief]

[2]. Kasahara Y, et al. Formation of a conformationally changed C1r, a subcomponent of the first component of human complement, as an intermediate of its autoactivation reaction. FEBS Lett. 1982;141(1):128-131. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3303 mL	16.6517 mL	33.3034 mL	83.2584 mL
	5 mM	0.6661 mL	3.3303 mL	6.6607 mL	16.6517 mL
	10 mM	0.3330 mL	1.6652 mL	3.3303 mL	8.3258 mL
	15 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	20 mM	0.1665 mL	0.8326 mL	1.6652 mL	4.1629 mL
	25 mM	0.1332 mL	0.6661 mL	1.3321 mL	3.3303 mL
	30 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL
	40 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0815 mL
	50 mM	0.0666 mL	0.3330 mL	0.6661 mL	1.6652 mL
	60 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3876 mL
	80 mM	0.0416 mL	0.2081 mL	0.4163 mL	1.0407 mL

MBD Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MBD33984-50-87-(p-Methoxybenzylamino)-4-nitrobenz-2,1,3-oxadiazoleFluorescent Dyeproteinnucleoproteinconformationbacterial ribosomestrypsinogentrypsinhydrophobicmacromoleculeInhibitorinhibitorinhibit

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