2. Academic Validation
  3. Sortin1-hypersensitive mutants link vacuolar-trafficking defects and flavonoid metabolism in Arabidopsis vegetative tissues

Sortin1-hypersensitive mutants link vacuolar-trafficking defects and flavonoid metabolism in Arabidopsis vegetative tissues

  • Chem Biol. 2011 Feb 25;18(2):187-97. doi: 10.1016/j.chembiol.2010.11.015.
Abel Rosado 1,  Glenn R Hicks,  Lorena Norambuena,  Ilana Rogachev,  Sagit Meir,  Lucille Pourcel,  Jan Zouhar,  Michelle Q Brown,  Marietta P Boirsdore,  Rachel S Puckrin,  Sean R Cutler,  Enrique Rojo,  Asaph Aharoni,  Natasha V Raikhel
Affiliations

Affiliation

  • 1 Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California-Riverside, CA 92521, USA.
PMID: 21338917 DOI: 10.1016/j.chembiol.2010.11.015
Abstract

Sortin1 is a chemical genetic-hit molecule that causes specific mislocalization of plant and yeast-soluble and membrane vacuolar markers. To better understand its mode of action, we designed a Sortin1-hypersensitive screen and identified several Sortin1-hypersensitive and flavonoid-defective mutants. Mechanistically, Sortin1 mimics the effect of the glutathione inhibitor buthionine sulfoximine and alters the vacuolar accumulation of Flavonoids, likely blocking their transport through vacuole-localized ABC transporters. Structure-activity relationship studies conducted in Arabidopsis revealed the structural requirements for Sortin1 bioactivity and demonstrated that overlapping Sortin1 substructures can be used to discriminate between vacuolar-flavonoid accumulations and vacuolar-biogenesis defects. We conclude that Sortin1 is a valuable probe for dissecting novel links among flavonoid transport, vacuolar integrity, and the trafficking of vacuolar targeted cargoes in Arabidopsis.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-12827
    99.04%, Vacuolar Protein Sorting Inhibitor