2. Academic Validation
  3. (-)-Hydroxycitric Acid Influenced Fat Metabolism via Modulating of Glucose-6-phosphate Isomerase Expression in Chicken Embryos

(-)-Hydroxycitric Acid Influenced Fat Metabolism via Modulating of Glucose-6-phosphate Isomerase Expression in Chicken Embryos

  • J Agric Food Chem. 2019 Jul 3;67(26):7336-7347. doi: 10.1021/acs.jafc.9b02330.
Shengnan Li 1 2,  Zhongmiao Yang 1 2,  Huihui Zhang 1 2,  Mengling Peng 3,  Haitian Ma 1 2
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine , Nanjing Agricultural University , Nanjing 210095 , China.
  • 2 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine , Nanjing Agricultural University , Nanjing 210095 , China.
  • 3 College of Animal Science and Technology , Anhui Agricultural University , Hefei 230036 , China.
PMID: 31184119 DOI: 10.1021/acs.jafc.9b02330
Abstract

The current research aimed to explore the impact of (-)-hydroxycitric acid (HCA) on fat metabolism and investigate whether this action of (-)-HCA was associated with modulation of glucose-6-phosphote isomerase (GPI) expression in chicken embryos. We constructed a recombinant plasmid (sh2-GPI) to inhibit GPI expression, and then embryos were treated with (-)-HCA. Results showed that (-)-HCA reduced lipid droplet accumulation, triglyceride content, and lipogenesis factors mRNA level and increased lipolysis factors mRNA expression, while this effect caused by (-)-HCA was markedly reversed when the chicken embryos were pretreated with sh2-GPI. (-)-HCA increased phospho (p)-acetyl-CoA carboxylase, enoyl-CoA hydratase short chain-1, carnitine palmitoyl transferase 1A, p-AMP-activated protein kinase, and peroxisome proliferators-activated receptor α protein expression, and this action of (-)-HCA also dispelled when the chicken embryos were pretreated with sh2-GPI. These data demonstrated that (-)-HCA decreased fat deposition via activation of the AMPK pathway, and the fat-reduction action of (-)-HCA was due to the increasing of GPI expression in chicken embryos.

Keywords

(−)-hydroxycitric acid; chicken embryos; fat metabolism; glucose-6-phosphote isomerase.

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