2. Academic Validation
  3. Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe

Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe

  • Anal Chem. 2020 Mar 17;92(6):4419-4426. doi: 10.1021/acs.analchem.9b05327.
Yue Xu 1,  Zhangshuang Deng 2,  Yue Shi 1,  Xin Chen 3,  Jingyuan Xu 1,  Suyun Zhong 1,  Youli Xiao 3,  Nai-Kei Wong 4,  Yiqing Zhou 1 3
Affiliations

Affiliations

  • 1 School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.
  • 2 Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, 443002, China.
  • 3 CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
  • 4 National Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, The Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China.
PMID: 32053360 DOI: 10.1021/acs.analchem.9b05327
Abstract

Cancer cells rely on fatty acid synthase (FASN), a key Enzyme for de novo biosynthesis of long chain fatty acids, to sustain their proliferative potential and drive invasion. Unfortunately, conventional FASN assays are technically inadequate for discerning otherwise elusive FASN activity in complex biological milieux, which has hindered progress in the functional study of FASN and development of its inhibitors. Here, we describe a chemical probe with unprecedented selectivity and sensitivity for the labeling of active FASN in living cells, thus demonstrating a new analytical modality for visualizing endogenous FASN activity and exploring opportunities for drug discovery.

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