2. Induced Disease Models Products GPCR/G Protein Neuronal Signaling
  3. Immunology and Inflammatory Disease Models Digestive System Disease Models Cholecystokinin Receptor
  4. Pancreatitis Models Pancreatic Disease Models
  5. Ceruletide

Ceruletide  (Synonyms: Caerulein; Cerulein; FI-6934)

Cat. No.: HY-A0190 Purity: 99.49%
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Cérulétide est un décapeptide et un récepteur des cholécystokinines puissant. Cérulétide est un agent cholécystocinétique sûr et efficace avec un effet spasmogène direct sur le muscle de la vésicule biliaire et les voies biliaires.

Ceruletid ist ein Decapeptid und ein potenter cholecystokinin receptor-Agonist. Ceruletid ist ein sicheres und wirksames Cholecystokinetikum mit einer direkten spasmogenen Wirkung auf den Gallenblasenmuskel und die Gallengänge.

Ceruletide is a decapeptide and a potent cholecystokinin receptor agonist. Ceruletide is a safe and effective cholecystokinetic agent with a direct spasmogenic effect on the gallbladder muscle and bile ducts.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ceruletide Chemical Structure

Ceruletide Chemical Structure

CAS No. : 17650-98-5

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100 μg USD 40 In-stock
500 μg USD 70 In-stock
1 mg USD 110 In-stock
5 mg USD 420 In-stock
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Customer Review

Based on 80 publication(s) in Google Scholar

Other Forms of Ceruletide:

Ceruletide Related Antibodies

Top Publications Citing Use of Products

77 Publications Citing Use of MCE Ceruletide

RT-PCR
WB

    Ceruletide purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2019 Nov 1;384(1):111606.  [Abstract]

    Decreased SPOP expression in cerulein-induced CP mice. Pancreatic SPOP, NF-κB/p65 and downstream proteins are determined through western blotting. Protein levels are normalized to the GAPDH loading control. Densitometry analysis is performed using the ImageJ software.

    Ceruletide purchased from MedChemExpress. Usage Cited in: Can J Physiol Pharmacol. 2019 Jan;97(1):15-22.  [Abstract]

    Expressions of Wnt/β-Catenin pathway-related genes in pancreatic and small intestinal tissues in each group (control, Caerulein, SKL2001+Caerulein, SKL2001+control). Relative mRNA expressions of Wnt1 (A, F), Wnt2 (B, G), β-catenin (C, H), c-Myc (D, I) and Axin-2 (E, J) in pancreatic and small intestinal tissues in each group detected by qRT-PCR.

    Ceruletide purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2018 Feb 15;10(2):402-410.  [Abstract]

    Cerulein exposure increases the protein expression of MD2, while ALR reduces the Cerulein-induced upregulation in MD2 protein expression.

    View All Cholecystokinin Receptor Isoform Specific Products:

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    CCKAR CCKBR

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ceruletide is a decapeptide and a potent cholecystokinin receptor agonist. Ceruletide is a safe and effective cholecystokinetic agent with a direct spasmogenic effect on the gallbladder muscle and bile ducts[1].

    IC50 & Target

    Cholecystokinin receptor[4]
    In Vitro

    Ceruletide is similar chemically and biologically to the human gastrointestinal hormones cholecystokinin-pancreozymin (CCK) and gastrin II. Ceruletide stimulates gallbladder contraction, pancreatic exocrine secretion, gastric secretion, and motility in the distal duodenum, jejunum, ileum and colon, while delaying gastric emptying and inhibiting motility in the proximal duodenum[1]. Ceruletide in supramaximal but not in physiological doses activates NF-kappaB/Rel in vitro. This activation may induce a self-defending genetic program before the onset of cellular injury, which may prevent higher degrees of damage of pancreatic acinar cells after secretagogue hyperstimulation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ceruletide Related Antibodies
    In Vivo

    Ceruletide can be used to induce pancreatitis models. When S35-labeled Ceruletide is administered by intramuscular injection to rats, rabbits, and mice, the radioactivity in the blood of rats and rabbits reaches its peak within 5 and 15 minutes, respectively, followed by a rapid decline. Acute toxicity studies in mice show that the intravenous LD50 value for Ceruletide is 1012 mg/kg[4][5].

    Induction of Acute Pancreatitis[6][7][8]
    Background
    Ceruletide acts on CCK receptors, which are often expressed on various species of pancreatic acinar cells. Ceruletide induces dysregulation of the production and secretion of digestive enzymes, leading to cytoplasmic vacuolization and the death of acinar cells, edema formation, and an infiltration of inflammatory cells into the pancreas.
    Specific Mmodeling Methods
    1. Mice: C57Bl/6n mice • 8-12 week-old
    Administration: Ceruletide 50 μg/kg • i.p. • 8 hourly, total 8 times;
    2. Mice: C57BL6/J mice • male • 6-8 week-old
    Administration: Ceruletide 100 μg/kg plus LPS (5 mg/kg, i.p. immediately after the last injection of Ceruletide) • i.p. • 10 hourly, total 10 times;
    or Ceruletide (50 μg/kg, 7 hourly, total 7 times) plus LPS (10 mg/kg, once) • i.p.
    Note
    (1) Cerulein induces rapid pancreatitis and rapid spontaneous recovery within one week in mammals. Therefore, mice are usually euthanized within 24 h after the first Ceruletide injection.
    Modeling Indicators
    Molecular changes: Increased serum amylase, serum lipase, TNF-α, and IL-1β level.
    Histology analysis: Pancreatic edema, inflammatory infiltration, and acinar cell necrosis (H&E staining).
    Correlated Product(s): Lipopolysaccharides (HY-D1056)
    Opposite Product(s): /

    Induction of Chronic Pancreatitis[9]
    Background
    Chronic pancreatitis (CP) model can be established by repeated injection of mice with Ceruletide. Histologic characteristics of chronic pancreatitis include inflammatory infiltrates, fibrosis, acinar cell atrophy, duct distortion, and squamous metaplasia of the duct epithelium.
    Specific Mmodeling Methods
    Mice: C57BL/6 • female • 6 week-old
    Administration: Ceruletide 50 μg/kg • i.p. • 3 days per week, for a total of 4 weeks.
    Note
    Mice were sacrificed 3 days after the last injection.
    Modeling Indicators
    Histology analysis: Glandular atrophy, infiltration of immune cells and distorted and/or blocked ducts.
    Correlated Product(s): Pevonedistat (HY-70062)
    Opposite Product(s): /

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    1352.41

    Formula

    C58H73N13O21S2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    {pGlu}-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2
    Sequence Shortening

    {pGlu}-QD-Y(SO3H)-TGWMDF-NH2
    Structure Classification
    Initial Source

    the skin of a tropical frog
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (73.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    DMF : 16.67 mg/mL (12.33 mM; Need ultrasonic)

    H2O : 2.5 mg/mL (1.85 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7394 mL 3.6971 mL 7.3942 mL
    5 mM 0.1479 mL 0.7394 mL 1.4788 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (1.85 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (1.85 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 30.3 mg/mL (22.40 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  Saline

      Solubility: 2 mg/mL (1.48 mM); Clear solution; Need ultrasonic and adjust pH to 12 with 1M NaOH

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    SDS (252 KB)

    COA (252 KB) HNMR (257 KB) LCMS (314 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [3]

    Dogs[3]
    All dogs undergo serum bile acid (SBA) stimulation with food (<5 kg/body weight [BW] 2 teaspoons, >5 kg BW 2 tablespoons) or 0.3 μg/kg BW Ceruletide IM, respectively, on consecutive days. A diet of moderate protein content and with an increased concentration of fiber is chosen to minimize metabolic complications such as hepatic encephalopathy. Before each test, the dogs are fasted for 12 hours. Blood samples are drawn at baseline, 60 and 120 minutes after feeding, and 20, 30, and 40 minutes postinjection, respectively. The blood samples are collected in plain tubes and left to clot; they are then centrifuged at 6,500 ×g for 1 minute, and the serum is used to measure SBA by a colorimetric test with endpoint determination[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMF / DMSO 1 mM 0.7394 mL 3.6971 mL 7.3942 mL 18.4855 mL
    DMF / DMSO 5 mM 0.1479 mL 0.7394 mL 1.4788 mL 3.6971 mL
    10 mM 0.0739 mL 0.3697 mL 0.7394 mL 1.8486 mL
    DMSO 15 mM 0.0493 mL 0.2465 mL 0.4929 mL 1.2324 mL
    20 mM 0.0370 mL 0.1849 mL 0.3697 mL 0.9243 mL
    25 mM 0.0296 mL 0.1479 mL 0.2958 mL 0.7394 mL
    30 mM 0.0246 mL 0.1232 mL 0.2465 mL 0.6162 mL
    40 mM 0.0185 mL 0.0924 mL 0.1849 mL 0.4621 mL
    50 mM 0.0148 mL 0.0739 mL 0.1479 mL 0.3697 mL
    60 mM 0.0123 mL 0.0616 mL 0.1232 mL 0.3081 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Ceruletide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ceruletide17650-98-5Caerulein Cerulein FI-6934FI6934FI 6934FI-6934Cholecystokinin ReceptorCCK ReceptorInhibitorinhibitorinhibit

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