Chlorin A-mediated photodynamic therapy induced apoptosis in human cholangiocarcinoma cells via impaired autophagy flux

Background: Photodynamic therapy (PDT) is a promising strategy for multiple cancers. Chlorin e6 and its derivative 13¹-[2'-(2-pyridyl)ethylamine] Chlorin e6 (Chlorin A) are effective photosensitizers, although their cytotoxic mechanisms have not yet been fully characterized.

Methods: Cell viability and Apoptosis were evaluated by CCK8 assay, TUNEL assay, and Annexin V/PI staining. The expression levels of different proteins were analyzed by Western blot analysis and immunofluorescence. The crosstalk between Autophagy, endoplasmic reticulum stress (ERS), and mitochondrial dysfunction was investigated using Reactive Oxygen Species (ROS) scavenger N-acetyl cysteine (NAC), PERK Inhibitor GSK2606414, Autophagy Inhibitor 3-MA, and mitochondrial stabilizer elamipretide. Furthermore, the extent of ROS production, lysosomal damage, Autophagy flux, and mitochondrial membrane potential (MMP) were tracked using established probes. An in vivo xenograft model of cholangiocarcinoma (CCA) was established in BALB/c-nude mice by inoculation with EGI-1 cells, and Chlorin A was administered topically or intravenously, followed by LIGHT irradiation.

Results: Chlorin A-PDT decreased the viability of CCA cells and induced Apoptosis. Intriguingly, Chlorin A-PDT promoted Autophagy via activation of ROS-induced ERS-related PERK/p-eif2α/CHOP axis, and blocked the ensuing Autophagy flux by lysosomal damage. The PERK Inhibitor GSK2606414 and NAC alleviated Apoptosis and Autophagy induced by Chlorin A-PDT. Furthermore, mitochondrial dysfunction aggravated ERS, and stabilizing the mitochondria reduced both Apoptosis and Autophagy. Finally, Chlorin A-PDT significantly reduced tumor growth in vivo.

Conclusions: Chlorin A-PDT induced Apoptosis in CCA cells by initiating Autophagy and impaired the Autophagy flux via ROS-mediated ERS and lysosomal damage.