Temoporfin (Synonyms: m-THPC; KW2345)

Name: Temoporfin
Brand: MedChemExpress
SKU: HY-16488
Rating: 5.0 (11 reviews)

Cat. No.: HY-16488 Purity: 97.10%: FAQs
Data Sheet SDS COA Handling Instructions

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Temoporfin (m-THPC), a reduced porphyrin, is a potent second-generation photosensitizer. Temoporfin can be used in the research of photodynamic therapy (PDT) for head and neck cancers.

For research use only. We do not sell to patients.

Temoporfin Chemical Structure

CAS No. : 122341-38-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 198	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 198	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 228	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 468	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 828	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 11 publication(s) in Google Scholar

10 Publications Citing Use of MCE Temoporfin

: Temoporfin purchased from MedChemExpress. Usage Cited in: Theranostics. 2018 Feb 4;8(5):1435-1448. [Abstract]; In vivo fluorescence images of glioma-bearing mice intravenously receiving different mTHPC-containing formulations.

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Description

Temoporfin (m-THPC), a reduced porphyrin, is a potent second-generation photosensitizer. Temoporfin can be used in the research of photodynamic therapy (PDT) for head and neck cancers^[1]^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	0.05 μM Compound: Temoporfin	Phototoxicity against mouse 4T1 cells assessed as reduction in cell viability preincubated for 4 hrs followed by irradiation with 0.3 W/cm2 of 625 +/- 2 nanometer LED light for 1 hr and measured after 42 hrs by MTT assay Phototoxicity against mouse 4T1 cells assessed as reduction in cell viability preincubated for 4 hrs followed by irradiation with 0.3 W/cm2 of 625 +/- 2 nanometer LED light for 1 hr and measured after 42 hrs by MTT assay	[PMID: 32916313]
4T1	IC₅₀	38.01 μM Compound: Temoporfin	Dark toxicity in mouse 4T1 cells assessed as reduction in cell viability by MTT assay Dark toxicity in mouse 4T1 cells assessed as reduction in cell viability by MTT assay	[PMID: 32916313]
A549	IC₅₀	0.702 μM Compound: m-THPC	Photodynamic cytotoxicity against human A549 cells assessed as reduction in cell viability preincubated for 24 hrs followed by compound washout and subsequent 650 nanometer laser light irradiation at 2 J/cm2 measured by MTT assay Photodynamic cytotoxicity against human A549 cells assessed as reduction in cell viability preincubated for 24 hrs followed by compound washout and subsequent 650 nanometer laser light irradiation at 2 J/cm2 measured by MTT assay	[PMID: 31132530]
A549	IC₅₀	0.779 μM Compound: m-THPC	Photodynamic cytotoxicity against human A549 cells assessed as reduction in cell viability preincubated for 24 hrs followed by compound washout and subsequent 650 nanometer laser light irradiation at 1 J/cm2 measured by MTT assay Photodynamic cytotoxicity against human A549 cells assessed as reduction in cell viability preincubated for 24 hrs followed by compound washout and subsequent 650 nanometer laser light irradiation at 1 J/cm2 measured by MTT assay	[PMID: 31132530]
A549	IC₅₀	1.099 μM Compound: m-THPC	Photodynamic cytotoxicity against human A549 cells assessed as reduction in cell viability preincubated for 24 hrs followed by compound washout and subsequent 650 nanometer laser light irradiation at 0.5 J/cm2 measured by MTT assay Photodynamic cytotoxicity against human A549 cells assessed as reduction in cell viability preincubated for 24 hrs followed by compound washout and subsequent 650 nanometer laser light irradiation at 0.5 J/cm2 measured by MTT assay	[PMID: 31132530]
A549	CC₅₀	40.7 μM Compound: 40	Cytotoxicity against human A549 cells incubated for 42 hrs by MTT assay Cytotoxicity against human A549 cells incubated for 42 hrs by MTT assay	[PMID: 34458734]
HUVEC	IC₅₀	> 100 μM Compound: Temoporfin	Dark toxicity in HUVEC assessed as reduction in cell viability by MTT assay Dark toxicity in HUVEC assessed as reduction in cell viability by MTT assay	[PMID: 32916313]
HUVEC	IC₅₀	1.5 μM Compound: Temoporfin	Phototoxicity against HUVEC assessed as reduction in cell viability preincubated for 4 hrs followed by irradiation with 0.3 W/cm2 of 625 +/- 2 nanometer LED light for 1 hr and measured after 42 hrs by MTT assay Phototoxicity against HUVEC assessed as reduction in cell viability preincubated for 4 hrs followed by irradiation with 0.3 W/cm2 of 625 +/- 2 nanometer LED light for 1 hr and measured after 42 hrs by MTT assay	[PMID: 32916313]
MDA-MB-231	IC₅₀	0.87 μM Compound: Temoporfin	Phototoxicity against human MDA-MB-231 cells assessed as reduction in cell viability preincubated for 4 hrs followed by irradiation with 0.3 W/cm2 of 625 +/- 2 nanometer LED light for 1 hr and measured after 42 hrs by MTT assay Phototoxicity against human MDA-MB-231 cells assessed as reduction in cell viability preincubated for 4 hrs followed by irradiation with 0.3 W/cm2 of 625 +/- 2 nanometer LED light for 1 hr and measured after 42 hrs by MTT assay	[PMID: 32916313]
MDA-MB-231	IC₅₀	33.42 μM Compound: Temoporfin	Dark toxicity in human MDA-MB-231 cells assessed as reduction in cell viability by MTT assay Dark toxicity in human MDA-MB-231 cells assessed as reduction in cell viability by MTT assay	[PMID: 32916313]

In Vitro

A detailed confocal fluorescence microscopy study of a human adenocarcinoma shows weak localization of Temoporfin in lysosomes and mitochondria. Instead, it is found to be localized in the endoplasmic reticulum (ER) and the Golgi apparatus^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Temoporfin in liver tissue decreases rapidly in time after initial high levels at 4 h after administration (0.1-0.3 mg/kg). In tumour tissue no decrease in photosensitizer levels occurred, with Temoporfin remaining high up to 48 h after administration^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

680.75

Formula

C₄₄H₃₂N₄O₄

CAS No.

122341-38-2

Appearance

Solid

Color

Purple to black

SMILES

OC1=CC=CC(/C2=C3CCC(/C(C4=CC(O)=CC=C4)=C5C=C/C(N/5)=C(C6=CC(O)=CC=C6)/C(C=C/7)=NC7=C(C8=CC(O)=CC=C8)/C9=CC=C2N9)=N\3)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 20.83 mg/mL (30.60 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4690 mL	7.3448 mL	14.6897 mL
5 mM	0.2938 mL	1.4690 mL	2.9379 mL
10 mM	0.1469 mL	0.7345 mL	1.4690 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.47 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (1.47 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.29%

Select Batch:

Data Sheet (284 KB) SDS (420 KB)

COA (261 KB) LCMS (260 KB)

Handling Instructions (2659 KB)

References

[1]. Senge MO, et, al. Temoporfin (Foscan®, 5,10,15,20-tetra(m-hydroxyphenyl)chlorin)--a second-generation photosensitizer. Photochem Photobiol. 2011 Nov-Dec;87(6):1240-96. [Content Brief]

[2]. Rovers JP, et, al. Effective treatment of liver metastases with photodynamic therapy, using the second-generation photosensitizer meta-tetra(hydroxyphenyl)chlorin (mTHPC), in a rat model. Br J Cancer. 1999 Oct;81(4):600-8. [Content Brief]

[3]. Reshetov V, et, al. Photodynamic therapy with conventional and PEGylated liposomal formulations of mTHPC (temoporfin): comparison of treatment efficacy and distribution characteristics in vivo. Int J Nanomedicine. 2013;8:3817-31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4690 mL	7.3448 mL	14.6897 mL	36.7242 mL
	5 mM	0.2938 mL	1.4690 mL	2.9379 mL	7.3448 mL
	10 mM	0.1469 mL	0.7345 mL	1.4690 mL	3.6724 mL
	15 mM	0.0979 mL	0.4897 mL	0.9793 mL	2.4483 mL
	20 mM	0.0734 mL	0.3672 mL	0.7345 mL	1.8362 mL
	25 mM	0.0588 mL	0.2938 mL	0.5876 mL	1.4690 mL
	30 mM	0.0490 mL	0.2448 mL	0.4897 mL	1.2241 mL

Temoporfin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Temoporfin122341-38-2m-THPC KW2345KW2345KW 2345KW-2345Reactive Oxygen Speciesreduced porphyrinphotosensitizerphotodynamic therapyPDThead and neck cancerInhibitorinhibitorinhibit

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Temoporfin (Synonyms: m-THPC; KW2345)

Customer Review

Temoporfin Related Antibodies

10 Publications Citing Use of MCE Temoporfin

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Temoporfin Related Antibodies

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Complete Stock Solution Preparation Table

Temoporfin Related Classifications

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