2. Academic Validation
  3. Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation

Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation

  • Vet Microbiol. 2021 Jan;252:108908. doi: 10.1016/j.vetmic.2020.108908.
Wenping Cui 1,  Jin Huang 2,  Xiangyun Niu 3,  Hongqi Shang 4,  Zhou Sha 5,  Yongqiang Miao 6,  Huan Wang 7,  Ruichang Chen 8,  Kai Wei 9,  Ruiliang Zhu 10
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China. Electronic address: sducwp@163.com.
  • 2 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 838844760@qq.com.
  • 3 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 1210114236@qq.com.
  • 4 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 870308726@qq.com.
  • 5 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 365787210@qq.com.
  • 6 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 451233165@qq.com.
  • 7 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 1535972552@qq.com.
  • 8 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China. Electronic address: 834522948@qq.com.
  • 9 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China. Electronic address: weikaisdau@163.com.
  • 10 College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271000, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China. Electronic address: zhurl@sdau.edu.cn.
PMID: 33254056 DOI: 10.1016/j.vetmic.2020.108908
Abstract

The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship. The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1. Under these conditions, extraction yield of total polysaccharide was 6.5 % ± 0.19 %. Total polysaccharide was then purified by DEAE-52 cellulose and Sephadex G-200 gel. Three fractions, PPP-1, PPP-2, and PPP-3, were identified with molecular weight of 463.70, 99.41, and 26.97 kDa, respectively. Three polysaccharide fractions were all composed of 10 Monosaccharides in different proportions. Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid. The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent. In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05). The Antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major Antiviral component. The structure-activity relationship showed that the Antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors. These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic Antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for Antiviral agent.

Keywords

Antiviral activity; Avian leukosis virus subgroup J; Pinus massoniana pollen; Structure activity relationship.

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