2. Academic Validation
  3. Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles

Targeting laryngeal cancer cells with 5-fluorouracil and curcumin using mesoporous silica nanoparticles

  • Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820962114. doi: 10.1177/1533033820962114.
Ding Wang 1 2,  Dan Yu 1,  Xueshibojie Liu 1,  Qian Wang 2,  Xuyang Chen 2,  Xindan Hu 2,  Qiong Wang 2,  Chunshun Jin 1,  Lianji Wen 1,  Ling Zhang 2
Affiliations

Affiliations

  • 1 Department of Otolaryngology, Head and Neck Surgery, the Second Hospital, 12510Jilin University, Changchun, Jilin, People's Republic of China.
  • 2 Department of Pathophysiology, College of Basic Medical Science, 12510Jilin University, Changchun, Jilin, People's Republic of China.
PMID: 33267716 DOI: 10.1177/1533033820962114
Abstract

Objective: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal Cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers.

Methods: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect Apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded.

Results: 5-Fluorouracil and curcumin synergistically induced Apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin.

Conclusions: Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal Cancer.

Keywords

5-fluorouracil; Laryngeal cancer; apoptosis; cell cycle arrest; curcumin; mesoporous silica nanoparticles.

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