2. Academic Validation
  3. MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway

MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway

  • J Cancer. 2021 Jan 1;12(2):467-473. doi: 10.7150/jca.49641.
Tonglei Chen 1,  Zhenshi Chen 2 3,  Xiaoning Lian 4,  Weidong Wu 2 3,  Lei Chu 2 3,  Shaoru Zhang 2 3,  Lihui Wang 2 3
Affiliations

Affiliations

  • 1 Suzhou Ninth People's Hospital, Suzhou, Jiangsu 215200, China.
  • 2 Danyang People's Hospital of Jiangsu Province, Danyang, Jiangsu 212300, China.
  • 3 Danyang Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, China.
  • 4 Department of Assay Development, TOT BIOPHARM co., LTD, Suzhou, Jiangsu 215024, China.
PMID: 33391443 DOI: 10.7150/jca.49641
Abstract

Objective: To investigate the high expression of MUC15 in promoting proliferation, migration and invasion in osteosarcoma (OS) cell and its potential mechanism. Methods: The expressions of MUC15 in OS patients were analyzed from GEO Datasets, tumor cell lines and clinical samples. The roles of MUC15 in OS were explored by CCK-8, flow cytometry, transwell and western blot assay, respectively. Results: MUC15 was highly expressed in osteosarcoma, and there was a significant negative correlation between MUC15 and the prognosis. Knockdown of MUC15 in HOS and U-2OS could promote tumor cell Apoptosis, down-regulate the expression of MMP2/9, reduce the epithelial interstitial transition and silence the Wnt/b-Catenin signal pathway. Conclusion: The high-expression of MUC15 promotes the proliferation, migration and invasion of osteosarcoma through anti-apoptosis, increasing the invasive ability by epithelial interstitial transition, and activating the Wnt/b-Catenin signal pathway.

Keywords

MUC 15; Osteosarcoma; invasion; migration; proliferation.

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