miR-130a-Mediated KLF3 Can Inhibit the Growth of Lung Cancer Cells

Background: The role of MicroRNA (miR) in tumors has been reported in numerous articles. Previous studies have found that miR-130a is low expressed in lung Cancer, but the related mechanism has not been fully elucidated. This study mainly explores the mechanism of miR-130a in lung Cancer, so as to provide potential therapeutic targets for clinical applications.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-130a and KLF3 in the tissues of lung Cancer patients. The miR-130a-mimics and miR-130a-inhibit were constructed. Cell proliferation, invasion, migration and Apoptosis were determined by CCK-8, transwell, scratch test and flow cytometry. Western Blot was used to determine the expression of KLF3 protein in cells, and the dual-luciferase reporter to determine the relationship between KLF3 and miR-130a.

Results: miR-130a shows low expression in NSCLC patients, while KLF3 shows high expression, exhibiting a negative correlation. The 5-year survival rate of patients with low miR-130a expression and high KLF3 expression was reduced. COX regression analysis showed that miR-130a was an independent prognostic factor for NSCLC patients. The dual-luciferase reporter revealed that miR-130a bound to KLF3 in a targeted manner, and cell experiments showed that miR-130a could inhibit the growth of lung Cancer cells by regulating the expression of KLF3.

Conclusion: miR-130a shows low expression in lung Cancer and predicts a poor prognosis. In addition, up-regulation of miR-130a can down-regulate KLF3 and inhibit the growth of lung Cancer.