2. Academic Validation
  3. Opuntia ficus-indica Extract and Isorhamnetin-3-O-Glucosyl-Rhamnoside Diminish Tumor Growth of Colon Cancer Cells Xenografted in Immune-Suppressed Mice through the Activation of Apoptosis Intrinsic Pathway

Opuntia ficus-indica Extract and Isorhamnetin-3-O-Glucosyl-Rhamnoside Diminish Tumor Growth of Colon Cancer Cells Xenografted in Immune-Suppressed Mice through the Activation of Apoptosis Intrinsic Pathway

  • Plant Foods Hum Nutr. 2021 Dec;76(4):434-441. doi: 10.1007/s11130-021-00934-3.
M Antunes-Ricardo # 1,  D Guardado-Félix # 1,  M R Rocha-Pizaña 2,  J Garza-Martínez 1,  L Acevedo-Pacheco 1,  J A Gutiérrez-Uribe 3 4,  J Villela-Castrejón 1,  F López-Pacheco 1,  S O Serna-Saldívar 5
Affiliations

Affiliations

  • 1 Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., Mexico.
  • 2 Tecnologico de Monterrey, Campus Puebla, Vía Atlixcáyotl 2301, C.P. 72453, Puebla, Puebla, Mexico.
  • 3 Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., Mexico. jagu@tec.mx.
  • 4 Tecnologico de Monterrey, Campus Puebla, Vía Atlixcáyotl 2301, C.P. 72453, Puebla, Puebla, Mexico. jagu@tec.mx.
  • 5 Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., Mexico. sserna@tec.mx.
  • # Contributed equally.
PMID: 34786663 DOI: 10.1007/s11130-021-00934-3
Abstract

This study aimed to evaluate the effects of Opuntia ficus-indica extract (OFI-E) and its glycoside isorhamnetin-3-O-glucosyl-rhamnoside (IGR) on the growth of human colorectal adenocarcinoma cells and in a xenografted-immunosuppressed mice model. The IC50 values of OFI-E and IGR on colon Cancer cells (HT-29 RFP) were determinate, as well as their effects on the cell cycle and Apoptosis induction. OFI-E and IGR produced an increased in Apoptosis induction, ROS production and a G0/G1 cell cycle arrest. In xenografted-inmunosupressed mice, OFI-E and IGR reduced the tumor growth rate, myeloperoxidase activity and total Cholesterol levels. OFI-E and IGR reduced the tumor growth through the overexpression of cleaved Caspase-9, HDAC11, and Bai1 proteins. OFI-E reduced the expression of Bcl-2. Results demonstrated the chemopreventive effects of OFI-E, and its purified compound IGR, showing their potential as an alternative in the treatment of colorectal Cancer.

Keywords

Apoptosis; Cell arrest; Colon cancer; Isorhamnetin; Opuntia ficus-indica; Xenograft.

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