2. Academic Validation
  3. Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay

Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay

  • Chem Asian J. 2022 May 16;17(10):e202200106. doi: 10.1002/asia.202200106.
Zhe Zheng 1,  Siying Ren 1,  Wen-Chao Geng 2,  Xuexian Cui 3 4,  Bian Wu 3,  Hong Wang 1
Affiliations

Affiliations

  • 1 School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou, Jiangsu, P. R. China.
  • 2 Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, P. R. China.
  • 3 CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, P. R. China.
  • 4 College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.
PMID: 35333438 DOI: 10.1002/asia.202200106
Abstract

Methionine is an essential amino acid involved in many physiological and pathological processes. Methionine starvation caused by methionine decarboxylase (MetDC) degradation becomes a promising strategy for Cancer treatment. Multistep colorimetric method, the present approach to monitor the MetDC activity, possesses drawbacks of the complicated process, low accuracy, and poor anti-interference due to indirect detection. Herein, we report a facile and easy-to-use supramolecular tandem assay (STA) with the cucurbit[7]uril and acridine orange reporter pair for the direct and real-time monitoring of MetDC activity. This strategy not only provides a feasible method for enzymatic activity detection but also establishes the capability of inhibitor screening.

Keywords

cucurbit[7]uril; enzyme inhibitor; fluorescence detection; methionine decarboxylase; supramolecular tandem assay.

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