1. Academic Validation
  2. Role of the hydrogen sulfide-releasing donor ADT-OH in the regulation of mammal neural precursor cells

Role of the hydrogen sulfide-releasing donor ADT-OH in the regulation of mammal neural precursor cells

  • J Cell Physiol. 2022 Jul;237(7):2877-2887. doi: 10.1002/jcp.30726.
Shan-Wen Wei 1 Ming-Ming Zou 2 Jian Huan 3 Di Li 4 Peng-Fei Zhang 2 Mei-Hong Lu 5 Jian Xiong 4 Yan-Xia Ma 1
Affiliations

Affiliations

  • 1 Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, Suzhou, China.
  • 2 Department of Neurosurgery, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • 3 Department of Radiation Oncology, The Affiliated Suzhou Science & Technology Town Hospital of Nanjing Medical University, Suzhou, China.
  • 4 Department of Rehabilitation, The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou, China.
  • 5 School of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Abstract

Neural precursor cells (NPCs) generate new neurons to supplement neuronal loss as well as to repair damaged neural circuits. Therefore, NPCs have potential applications in a variety of neurological diseases, such as spinal cord injury, traumatic brain injury, and glaucoma. Specifically, improving NPCs proliferation and manipulating their differentiated cell types can be a beneficial therapy for a variety of these diseases. ADT-OH is a slow-releasing organic H2 S donor that produces a slow and continuous release of H2 S to maintain normal brain functions. In this study, we aimed to explore the effect of ADT-OH on NPCs. Our results demonstrated that ADT-OH promotes self-renewal and antiapoptosis ability of cultured NPCs. Additionally, it facilitates more NPCs to differentiate into neurons and oligodendrocytes, while inhibiting their differentiation into astrocytes. Furthermore, it enhances axonal growth. Moreover, we discovered that the mRNA and protein expression of β-catenin, TCF7L2, c-Myc, Ngn1, and Ngn2, which are key genes that regulate NPCs self-renewal and differentiation, were increased in the presence of ADT-OH. Altogether, these results indicate that ADT-OH may be a promising drug to regulate the neurogenesis of NPCs, and needs to be studied in the future for clinical application potential.

Keywords

ADT-OH; NPCs; directional differentiation; proliferation; survive.

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