ADT-OH (Synonyms: 5-(4-Hydroxyphenyl)-3H-1,2-dithiole-3-thione; ACS 1)

Name: ADT-OH
Brand: MedChemExpress
SKU: HY-109582
Rating: 5.0 (2 reviews)

Cat. No.: HY-109582 Purity: 99.06%: FAQs
Data Sheet SDS COA Handling Instructions

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ADT-OH is a hydrogen sulfide-releasing donor. ADT-OH induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD. ADT-OH has the potential for the research of cancer diseases.

For research use only. We do not sell to patients.

ADT-OH Chemical Structure

CAS No. : 18274-81-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE ADT-OH

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: ADT-OH	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31202992]
A549	IC₅₀	66 μM Compound: ADTOH	Antiproliferative activity against human A549 cells after 3 days Antiproliferative activity against human A549 cells after 3 days	[PMID: 18294844]
A549	IC₅₀	66 μM Compound: ADTOH	Antiproliferative activity against human A549 cells after 48 hrs Antiproliferative activity against human A549 cells after 48 hrs	[PMID: 20576572]
B16	IC₅₀	> 50 μM Compound: 6; ADT-OH	Antiproliferative activity against mouse B16 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against mouse B16 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31877536]
Bel-7402	IC₅₀	> 50 μM Compound: ADT-OH	Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31202992]
Bel-7402	IC₅₀	> 50 μM Compound: ADTOH; 5	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 29635169]
Caco-2	IC₅₀	35.47 μM Compound: ADTOH; 5	Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay	[PMID: 29635169]
HCT-116	IC₅₀	> 50 μM Compound: 6; ADT-OH	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31877536]
HeLa	IC₅₀	0.45 μM Compound: ADTOH	Inhibition of HDAC in human HeLa cells extracts after 60 mins by fluorescence assay Inhibition of HDAC in human HeLa cells extracts after 60 mins by fluorescence assay	[PMID: 20576572]
HepG2	IC₅₀	> 50 μM Compound: 6; ADT-OH	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31877536]
HL-60	IC₅₀	25.9 μM Compound: ADTOH; 5	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 29635169]
HT-29	IC₅₀	35 μM Compound: ADT-OH	Cytotoxicity against human HT-29 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 24273639]
K562	IC₅₀	> 50 μM Compound: 6; ADT-OH	Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31877536]
K562	IC₅₀	> 50 μM Compound: ADT-OH	Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31202992]
L02	IC₅₀	> 50 μM Compound: 6; ADT-OH	Cytotoxicity against human L02 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human L02 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 31877536]
L02	IC₅₀	> 50 μM Compound: ADT-OH	Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31202992]
MCF7	IC₅₀	> 50 μM Compound: 6; ADT-OH	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31877536]
MCF7	IC₅₀	> 50 μM Compound: ADTOH; 5	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 29635169]
PBMC	IC₅₀	> 100 μM Compound: ADTOH; 5	Antiproliferative activity against human PBMC cells after 72 hrs by MTT assay Antiproliferative activity against human PBMC cells after 72 hrs by MTT assay	[PMID: 29635169]
PBMC	IC₅₀	> 50 μM Compound: 6; ADT-OH	Cytotoxicity against human PBMC cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human PBMC cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 31877536]
PBMC	IC₅₀	> 50 μM Compound: ADT-OH	Cytotoxicity against human PBMC assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human PBMC assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31202992]
SGC-7901	IC₅₀	> 50 μM Compound: ADT-OH	Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 31202992]
SGC-7901	IC₅₀	> 50 μM Compound: ADTOH; 5	Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 29635169]

Molecular Weight

226.34

Formula

C₉H₆OS₃

CAS No.

18274-81-2

Appearance

Solid

Color

Yellow to brown

SMILES

S=C1SSC(C2=CC=C(O)C=C2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (552.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4181 mL	22.0907 mL	44.1813 mL
5 mM	0.8836 mL	4.4181 mL	8.8363 mL
10 mM	0.4418 mL	2.2091 mL	4.4181 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.06%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (248 KB) HNMR (256 KB) RP-HPLC (278 KB) MS (179 KB)

Handling Instructions (2659 KB)

References

[1]. Cai F, et al. ADT-OH, a hydrogen sulfide-releasing donor, induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD. Cell Death Dis. 2020;11(1):33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4181 mL	22.0907 mL	44.1813 mL	110.4533 mL
	5 mM	0.8836 mL	4.4181 mL	8.8363 mL	22.0907 mL
	10 mM	0.4418 mL	2.2091 mL	4.4181 mL	11.0453 mL
	15 mM	0.2945 mL	1.4727 mL	2.9454 mL	7.3636 mL
	20 mM	0.2209 mL	1.1045 mL	2.2091 mL	5.5227 mL
	25 mM	0.1767 mL	0.8836 mL	1.7673 mL	4.4181 mL
	30 mM	0.1473 mL	0.7364 mL	1.4727 mL	3.6818 mL
	40 mM	0.1105 mL	0.5523 mL	1.1045 mL	2.7613 mL
	50 mM	0.0884 mL	0.4418 mL	0.8836 mL	2.2091 mL
	60 mM	0.0736 mL	0.3682 mL	0.7364 mL	1.8409 mL
	80 mM	0.0552 mL	0.2761 mL	0.5523 mL	1.3807 mL
	100 mM	0.0442 mL	0.2209 mL	0.4418 mL	1.1045 mL

ADT-OH Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ADT-OH18274-81-25-(4-Hydroxyphenyl)-3H-1,2-dithiole-3-thione ACS 1ACS1ACS 1ACS-1Apoptosishydrogen sulfide-releasingapoptosismelanomaFADDcancerInhibitorinhibitorinhibit

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ADT-OH (Synonyms: 5-(4-Hydroxyphenyl)-3H-1,2-dithiole-3-thione; ACS 1)

Customer Review

ADT-OH Related Antibodies

2 Publications Citing Use of MCE ADT-OH

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ADT-OH Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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