2. Academic Validation
  3. CircTFF1 Promotes Proliferation, Migration and Invasion of Lung Cancer Cells by Facilitating Methylation of BCL6B Promoter via miR-29c-3p/DNMT3A Axis

CircTFF1 Promotes Proliferation, Migration and Invasion of Lung Cancer Cells by Facilitating Methylation of BCL6B Promoter via miR-29c-3p/DNMT3A Axis

  • Mol Biotechnol. 2022 Nov 14. doi: 10.1007/s12033-022-00594-x.
Lantao Zhao 1 2,  Hongya Xie 3,  Peng Li 1,  Haisheng Chen 4,  Jiang He 5,  Liang Wang 2,  Yonghong Wang 2,  Bin Ni 6
Affiliations

Affiliations

  • 1 Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China.
  • 2 Department of Thoracic Surgery, The Second People's Hospital of Lianyungang City, Lianyungang, 222002, Jiangsu, China.
  • 3 Department of Thoracic Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 215008, Jiangsu, China.
  • 4 Department of Thoracic Surgery, The Affiliated Haian Hospital of Nantong University, Nantong, 226699, Jiangsu, China.
  • 5 Department of Thoracic Surgery, Wuzhong People's Hospital of Suzhou City, Suzhou, 215128, Jiangsu, China.
  • 6 Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China. 13358056120@189.cn.
PMID: 36376550 DOI: 10.1007/s12033-022-00594-x
Abstract

Lung Cancer exists as a major risk of cancer-related death worldwide. As a novel circular RNA (circRNA), circTFF1 has not been explored in lung Cancer thoroughly. This study aims to probe into the function and mechanism of circTFF1 in lung Cancer. RT-qPCR and western blot were used to detect expression of RNAs and proteins. The influence of circTFF1 and BCL6B on cells behavior was explored via CCK-8 assay, colony formation assay, wound healing assay and transwell assay. Methylation of BCL6B was detected via MSP assay. Bioinformatics analysis and mechanism assays were conducted for investigating the interaction among genes. CircTFF1 was found to be upregulated in lung Cancer cell lines used in this study. Functional experiments revealed circTFF1 facilitated cell proliferation, migration and invasion. BCL6B was downregulated in lung Cancer cell lines used in this study, and the downregulation of BCL6B was mainly mediated by methylation. Additionally, BCL6B exerted suppressive impacts on lung Cancer cell line malignant behavior. Moreover, circTFF1 acted as the miR-29c-3p Sponge to upregulate DNMT3A, which facilitated the methylation of BCL6B. In all, circTFF1 promoted proliferation, migration and invasion of lung Cancer cell lines by facilitating methylation of BCL6B promoter via miR-29c-3p/DNMT3A axis.

Keywords

BCL6B; CircTFF1; DNMT3A; Lung cancer; Methylation.

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