2. Academic Validation
  3. Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

  • Mater Today Bio. 2022 Dec 5;18:100512. doi: 10.1016/j.mtbio.2022.100512.
Zhanqiu Dai 1 2 3 4,  Chen Xia 2 3 4,  Tingxiao Zhao 4,  Haoli Wang 2 3,  Hongsen Tian 2 3,  Ouyuan Xu 5,  Xunbin Zhu 1,  Jun Zhang 4,  Pengfei Chen 2 3 6
Affiliations

Affiliations

  • 1 Department of Orthopaedics, The Second Affiliated Hospital of Bengbu Medical College, Anhui, China.
  • 2 Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
  • 3 Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, China.
  • 4 Department of Spine Surgery, Zhejiang Provincial People's Hospital, Hangzhou Medical College People's Hospital, Hangzhou, Zhejiang, China.
  • 5 College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • 6 Hangzhou OrigO Biotechnology Co. Ltd., Hangzhou, Zhejiang, China.
PMID: 36536658 DOI: 10.1016/j.mtbio.2022.100512
Abstract

Mitochondrial dysfunction causes the production of Reactive Oxygen Species (ROS) and oxidative damage, and oxidative stress and inflammation are considered key factors causing intervertebral disc degeneration (IVDD). Thus, restoring the mitochondrial dysfunction is an attractive strategy for treating IVDD. Platelet-derived extracellular vesicles (PEVs) are nanoparticles that target inflammation. Moreover, the vesicles produced by platelets (PLTs) have considerable anti-inflammatory effects. We investigate the use of PEVs as a therapeutic strategy for IVDD in this study. We extract PEVs and evaluate their properties; test their effects on H2O2-induced oxidative damage of nucleus pulposus (NP) cells; verify the role of PEVs in repairing H2O2-induced cellular mitochondrial dysfunction; and demonstrate the therapeutic effects of PEVs in a rat IVDD model. The results confirm that PEVs can restore impaired mitochondrial function, reduce oxidative stress, and restore cell metabolism by regulating the Sirtuin 1 (SIRT1)-peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α)-mitochondrial transcription factor A (TFAM) pathway; in rat models, PEVs retard the progression of IVDD. Our results demonstrate that the injection of PEVs can be a promising strategy for treating patients with IVDD.

Keywords

Intervertebral disc degeneration; Mitochondrial dysfunction; Nucleus pulposus; Platelets; Reactive oxygen species; Vesicles.

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