Prostaglandin E1 (Synonyms: Alprostadil; PGE1)

Name: Prostaglandin E1
Brand: MedChemExpress
SKU: HY-B0131
Rating: 5.0 (11 reviews)

Cat. No.: HY-B0131 Purity: 98.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Prostaglandin E1 (Alprostadil) is a prostanoid receptor ligand, with K_is of 1.1 nM, 2.1 nM, 10 nM, 33 nM and 36 nM for mouse EP3, EP4, EP2, IP and EP1, respectively. Prostaglandin E1 induces vasodilation and inhibits platelet aggregation. Prostaglandin E1 can be used as a vasodilator for the research of peripheral vascular diseases.

For research use only. We do not sell to patients.

Prostaglandin E1 Chemical Structure

CAS No. : 745-65-3

Size	Price	Stock	Quantity
1 mg	USD 37	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 79	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 119	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 248	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 373	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 560	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Prostaglandin E1:

11 Publications Citing Use of MCE Prostaglandin E1

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

EP	Human Endogenous Metabolite

In Vitro

Prostaglandin E1 (1 nM-10 μM; 48 h) concentration-dependently reduces HUVECs proliferation (up to 100% inhibition) in the presence of VEGF (20 ng/mL), with an IC₅₀ of 400 nM^[2].
Prostaglandin E1 (0.01-10 μM; 6 h) inhibits VEGF-induced HUVECs migration in a concentration dependent manner, with an IC₅₀ of 500 nM^[2].
Prostaglandin E1 (1-5 μM; 12-18 h) inhibits in vitro angiogenesis^[2].
Prostaglandin E1 (0.01-10 μM; 20 min) increases intracellular cAMP levels in HUVECs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Prostaglandin E1 (20 ng/animal/day; s.c. for 4 days) significantly inhibits the FGF-induced angiogenesis in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57/bl6 female mice (6-8 weeks) were injected with Matrigel supplemented with aFGF and heparin^[2]
Dosage:	20 ng/day/animal
Administration:	Minipump placed subcutaneously for 4 days
Result:	Visibly reduced the neovascularization process.

Clinical Trial

Molecular Weight

354.48

Formula

C₂₀H₃₄O₅

CAS No.

745-65-3

Appearance

Solid

Color

White to off-white

SMILES

CCCCC[C@H](O)/C=C/[C@@H]1[C@H](C(C[C@H]1O)=O)CCCCCCC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (282.10 mM; Need ultrasonic)

DMSO : 100 mg/mL (282.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8210 mL	14.1052 mL	28.2103 mL
5 mM	0.5642 mL	2.8210 mL	5.6421 mL
10 mM	0.2821 mL	1.4105 mL	2.8210 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.81%

Select Batch:

Data Sheet (277 KB) SDS (420 KB)

COA (250 KB) HNMR (255 KB) RP-HPLC (215 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Kiriyama M, et, al. Ligand binding specificities of the eight types and subtypes of the mouse prostanoid receptors expressed in Chinese hamster ovary cells. Br J Pharmacol. 1997 Sep;122(2):217-24. [Content Brief]

[2]. Cattaneo MG, et, al. Alprostadil suppresses angiogenesis in vitro and in vivo in the murine Matrigel plug assay. Br J Pharmacol. 2003 Jan;138(2):377-85. [Content Brief]

[3]. Hauck EW, et, al. Prostaglandin E1 long-term self-injection programme for treatment of erectile dysfunction--a follow-up of at least 5 years. Andrologia. 1999;31 Suppl 1:99-103. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.8210 mL	14.1052 mL	28.2103 mL	70.5258 mL
	5 mM	0.5642 mL	2.8210 mL	5.6421 mL	14.1052 mL
	10 mM	0.2821 mL	1.4105 mL	2.8210 mL	7.0526 mL
	15 mM	0.1881 mL	0.9403 mL	1.8807 mL	4.7017 mL
	20 mM	0.1411 mL	0.7053 mL	1.4105 mL	3.5263 mL
	25 mM	0.1128 mL	0.5642 mL	1.1284 mL	2.8210 mL
	30 mM	0.0940 mL	0.4702 mL	0.9403 mL	2.3509 mL
	40 mM	0.0705 mL	0.3526 mL	0.7053 mL	1.7631 mL
	50 mM	0.0564 mL	0.2821 mL	0.5642 mL	1.4105 mL
	60 mM	0.0470 mL	0.2351 mL	0.4702 mL	1.1754 mL
	80 mM	0.0353 mL	0.1763 mL	0.3526 mL	0.8816 mL
	100 mM	0.0282 mL	0.1411 mL	0.2821 mL	0.7053 mL

Prostaglandin E1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Prostaglandin E1 (Synonyms: Alprostadil; PGE1)

Customer Review

Other Forms of Prostaglandin E1:

Prostaglandin E1 Related Antibodies

11 Publications Citing Use of MCE Prostaglandin E1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Prostaglandin Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Prostaglandin E1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Prostaglandin E1 Related Classifications

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