2. Academic Validation
  3. Protective benefits of salvianic acid A against retinal iron overload by inhibition of ferroptosis

Protective benefits of salvianic acid A against retinal iron overload by inhibition of ferroptosis

  • Biomed Pharmacother. 2023 Jul 8;165:115140. doi: 10.1016/j.biopha.2023.115140.
Ying Zhao 1,  Qiang Li 1,  Wenyuan Jian 1,  Xue Han 2,  Yuanyuan Zhang 2,  Yan Zeng 3,  Rong Liu 3,  Qun Wang 4,  Qiongtao Song 5
Affiliations

Affiliations

  • 1 Eye School of Chengdu University of TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China; Ineye Hospital of Chengdu University of TCM, No.8 Xinghui Road, Chengdu 610084 Sichuan, China; Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection with TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China; Guangzhou Ineye Vision Health Innovation Institute, No.2 Fenghuang 3rd Road, Guangzhou 510555 Guangdong, China.
  • 2 Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, No.326 Xinshi South Road, Shijiazhuang 050200 Hebei, China.
  • 3 Eye School of Chengdu University of TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China.
  • 4 Eye School of Chengdu University of TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China; Ineye Hospital of Chengdu University of TCM, No.8 Xinghui Road, Chengdu 610084 Sichuan, China; Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection with TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China.
  • 5 Eye School of Chengdu University of TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China; Ineye Hospital of Chengdu University of TCM, No.8 Xinghui Road, Chengdu 610084 Sichuan, China; Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection with TCM, No.37 Twelve Bridge Road, Chengdu 610075 Sichuan, China; Guangzhou Ineye Vision Health Innovation Institute, No.2 Fenghuang 3rd Road, Guangzhou 510555 Guangdong, China. Electronic address: songqt607@cdutcm.edu.cn.
PMID: 37429233 DOI: 10.1016/j.biopha.2023.115140
Abstract

Background: Both the accumulation of Reactive Oxygen Species (ROS) and iron overload are significant variables that enhance the incidence of photoreceptor cell death and retinal degeneration. The discovery of Ferroptosis, which is characterized by iron-dependent lipid peroxidation, has led to a new perspective on how retinal degeneration develops. As a natural phenolic acid, salvianic acid A (SAA) from Salvia miltiorrhiza has promise in treating eye diseases. The purpose of this research was to learn more about SAA and its function in the development of iron-overload-induced retinal degeneration.

Methods: Models of iron overload in Kunming mice and the murine photoreceptor cell line 661 W were established, then the protective and antiferroptotic properties of SAA were assessed in vivo and in vitro.

Results: Biochemical and histopathological findings on the retina confirmed that SAA successfully alleviated retinal injury. In photoreceptor cells, iron overload caused cell death, mitochondrial dysfunction, ROS generation, and iron deposition. Salvianic acid A relieved lipid peroxidation and decreased iron accumulation by modulating Acyl-CoA synthetase long-chain family member 4, Glutathione Peroxidase 4, solute carrier family 7 member 11, and iron-metabolism-related proteins. The mitochondrial morphology suggests that the retinal protective effect of SAA is mediated via antiferroptotic action.

Conclusion: Ferroptosis plays an important role in the pathogenesis of iron-overload-induced retinal degeneration. New roles of SAA in Ferroptosis prevention via iron deposit inhibition, lipid peroxidation inhibition, and mitochondrial dysfunction reduction, were identified.

Keywords

Ferroptosis; Iron accumulation; Lipid peroxidation; Mitochondrion; Retinal degeneration; Salvianic acid A.

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