Photothermal theranostics with glutathione depletion and enhanced reactive oxygen species generation for efficient antibacterial treatment

Drug-resistant bacteria caused by the abuse of Antibiotics have brought great challenges to antimicrobial therapy. Herein an antibiotic-free polydopamine (PDA) modified metal-organic framework (PDA-FDM-23) with photothermal-enhanced chemodynamic effect was developed for synergistic Antibacterial treatment. The PDA-FDM-23 Antibacterial agent exhibited high peroxidase-like activity. Moreover, the process was significantly accelerated by consuming glutathione (GSH) to generate more efficient oxidizing Cu⁺. In addition, the photothermal therapy (PTT) derived from PDA improved the chemodynamic therapy (CDT) activity triggering a Reactive Oxygen Species explosion. This PTT-enhanced CDT strategy illustrated 100% Antibacterial efficiency against both Staphylococcus aureus and Escherichia coli. Cytotoxicity and hemolysis analyses fully demonstrated the excellent biocompatibility of PDA-FDM-23. Overall, our work highlighted the strong peroxidase catalytic activity, excellent GSH consumption and photothermal performance of PDA-FDM-23, providing a new strategy for antibiotic-free Reactive Oxygen Species (ROS) synergistic sterilization.