2. Academic Validation
  3. Protective Mechanisms of Various Active Substances on Cell DNA Damage and Apoptosis Induced by Deoxynivalenol

Protective Mechanisms of Various Active Substances on Cell DNA Damage and Apoptosis Induced by Deoxynivalenol

  • J Agric Food Chem. 2024 Mar 27;72(12):6651-6659. doi: 10.1021/acs.jafc.3c09100.
Silu Hou 1 2,  Jingjiao Ma 1,  Yuqiang Cheng 1,  Zhaofei Wang 1,  Hengan Wang 1,  Jian-He Sun 1,  Guiping Wang 3,  Aiqing Jia 3,  Ya-Xian Yan 1
Affiliations

Affiliations

  • 1 Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China.
  • 2 State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Demonstration Center of Food Quality and Safety Testing Technology, Tianjin University of Science and Technology, Tianjin 300457, China.
  • 3 Guangdong Haid Group Co. Limited, Guangzhou, Guangdong 511400, China.
PMID: 38501756 DOI: 10.1021/acs.jafc.3c09100
Abstract

Deoxynivalenol (DON) is a secondary metabolite of fungi that is harmful to humans and Animals. This study examined the protective effects of natural substances, including resveratrol, quercetin, vitamin E, vitamin C, and microbe-derived Antioxidants (MA), on both human gastric mucosal cells (GES-1) and pig small intestinal epithelial cells (IPEC-1) when induced by DON. Cells were incubated with active substances for 3 h and then exposed to DON for 24 h. The oxidative stress index, cell cycle, and Apoptosis were measured. As compared to cells treated only with DON, pretreatment with active substances improved the balance of the redox status in cells caused by DON. Specifically, quercetin, vitamin E, vitamin C, and MA showed the potential to alleviate the G2 phase cell cycle arrest effect that was induced by DON in both kinds of cells. It was observed that vitamin E and vitamin C can alleviate DON-induced Apoptosis and the G2 phase cycle arrest effect mediated via the ATM-Chk 2-Cdc 25C and ATM-P53 signaling pathways in GES-1 cells. In IPEC-1 cells, vitamin C and MA can alleviate both DON-induced Apoptosis and the G2 phase cycle arrest effect via the ATM-Chk 2-Cdc 25C signaling pathway. Different bioactive substances utilize different protective mechanisms against DON in interacting with different cells. The proper addition of vitamin E and vitamin C to food can neutralize the toxic effect of DON, while the addition of vitamin C and MA to animal feed can reduce the harm DON does to Animals.

Keywords

apoptosis; cycle arrest; deoxynivalenol; microbe-derived antioxidants; quercetin; vitamins.

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