2. Academic Validation
  3. LncRNA WFDC21P interacts with SEC63 to promote gastric cancer malignant behaviors by regulating calcium homeostasis signaling pathway

LncRNA WFDC21P interacts with SEC63 to promote gastric cancer malignant behaviors by regulating calcium homeostasis signaling pathway

  • Cancer Cell Int. 2024 Mar 25;24(1):111. doi: 10.1186/s12935-024-03297-2.
Jinyao Dong # 1,  Yongqiang Lv # 2,  Debin Meng # 1,  Ruyi Shi 3,  Feng Li 4,  Rui Guo 1,  Yi Wang 1,  Jiansheng Guo 5,  Yanyan Zhang 6
Affiliations

Affiliations

  • 1 Hepatobiliary Pancreatogastric Surgery, Shanxi Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University , Taiyuan, Shanxi, 030013, P. R. China.
  • 2 Scientific Research Department, Shanxi Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, 030013, P. R. China.
  • 3 Department of Cell biology and Genetics, Shanxi Medical University, Taiyuan, Shanxi, 030001, P. R. China.
  • 4 Central Laboratory, Shanxi Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, 030013, P. R. China.
  • 5 Gastrointestinal Surgery Department, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, P. R. China. jianshengguo@sina.com.
  • 6 Hepatobiliary Pancreatic Surgery and Liver Transplantation Center, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, P. R. China. zyy_2641@126.com.
  • # Contributed equally.
PMID: 38528582 DOI: 10.1186/s12935-024-03297-2
Abstract

Background: Gastric Cancer is currently estimated to be the fifth leading common Cancer in the world, and responsible for about one million new cases and an estimated 769,000 cancer-related deaths each year. WFDC21P is long non-coding RNA and has been reported to play critical roles in serval types of Cancer. Our research aims to investigate the biological effects and molecular mechanism of WFDC21P in gastric Cancer.

Methods: Datasets (GSE53137, GSE58828, and GSE109476) in GEO database were used to screen differential expressed lncRNAs in gastric Cancer by online GEO2R analysis tool. Quantitative RT-PCR was used to verify the above prediction in ten pairs of gastric Cancer and corresponding paracancerous tissues. Pan-cancer analysis was used to analyze the expression of WFDC21P in different types of Cancer. Small interfering RNAs were used to WFDC21P knockdown. CCK-8 and colony formation assays were used to measure the proliferation and tumorigenesis abilities. Wound healing and Transwell assay were used to detect the migration and invasion abilities. Proteins that interact with WFDC21P were predicted by catRAPID database. RNA pull down and RNA Immunoprecipitation were used to confirm the interaction. Western blotting was used to detect the key proteins level in calcium homeostasis signaling pathway. Loss-of-function and rescue assays were used to evaluate the biological function of SEC63 at the background of WFDC21P silencing.

Results: WFDC21P was upregulated in gastric Cancer tissues and cell lines. WFDC21P downregulation suppressed proliferation, tumorigenesis, migration, invasion, and promoted Apoptosis in gastric Cancer. SEC63 protein had the capability to bind with WFDC21P and the expression of SEC63 was regulated by WFDC21P. SEC63 was also upregulated in gastric Cancer and exerted effects during tumor growth and metastasis.

Conclusions: This study confirmed that lncRNA WFDC21P aggravated gastric Cancer malignant behaviors by interacting with SEC63 to regulate the calcium homeostasis signaling pathway.

Keywords

Calcium homeostasis; Gastric cancer; LncRNAs; SEC63; WFDC21P.

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