2. Academic Validation
  3. Sequence-dependent effects of hematoporphyrin derivatives (HPD) photodynamic therapy and cisplatin on lung adenocarcinoma cells

Sequence-dependent effects of hematoporphyrin derivatives (HPD) photodynamic therapy and cisplatin on lung adenocarcinoma cells

  • Photodiagnosis Photodyn Ther. 2024 Apr 26:104102. doi: 10.1016/j.pdpdt.2024.104102.
Nana Li 1,  Shichao Cui 2,  Aizhen Yang 3,  Baohong Xiao 4,  Yiwei Cao 5,  Xiaohui Yang 6,  Cunzhi Lin 7
Affiliations

Affiliations

  • 1 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: lnn112426@163.com.
  • 2 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: qdcuishichao@163.com.
  • 3 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: yangaizhen1985@126.com.
  • 4 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: xiao-1997@outlook.com.
  • 5 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: cyw13198@163.com.
  • 6 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: yangxiaohui@qdu.edu.cn.
  • 7 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.. Electronic address: lindoc@126.com.
PMID: 38679153 DOI: 10.1016/j.pdpdt.2024.104102
Abstract

Background: Hematoporphyrin derivatives (HPD)-Photodynamic therapy (PDT) in combination with cisplatin (DDP) is an effective Anticancer strategy. However, whether the order of combination affects efficacy has not been studied.

Methods: The human lung adenocarcinoma (LUAD) A549 cells were used as the study subjects. After A549 cells were treated with a single medication (PDT/DDP) or a sequential combination (PDT + DDP / DDP + PDT), the cell viability was assayed using the cell counting kit-8 method. Hoechst staining, Annexin-V/propidium iodide (PI) double staining, western blotting, and a real-time quantitative polymerase chain reaction (RT-qPCR) were performed to examine the mechanisms behind the combined effects.

Results: A synergistic impact between HPD-PDT and DDP was found. The cell viability in the PDT+DDP group was significantly lower than in the DDP+PDT group. A significant apoptotic profile and a high apoptotic rate were seen in the PDT + DDP group. The western blot showed that the expression levels of Bcl2-associated x(Bax) and cleaved-poly ADP-ribose polymerase (PARP) increased, and those of B-cell lymphoma-2 (Bcl-2) and Caspase-9 decreased in the PDT + DDP group. At the same time, the RT-qPCR revealed the upregulation of Bax and PARP mRNA and the downregulation of Bcl-2 and Caspase-9 mRNA.

Conclusion: The order of the combination therapy (PDT + DDP / DDP + PDT) was important. The HPD-PDT followed by DDP significantly inhibited LUAD cell viability, which may be related to the mitochondrial apoptotic pathway.

Keywords

Apoptosis; Cisplatin; Combined therapy; Hematoporphyrin derivatives; Photodynamic therapy.

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