2. Academic Validation
  3. Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection

Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection

  • Microbiol Res. 2024 May 15:285:127750. doi: 10.1016/j.micres.2024.127750.
Mianhuan Li 1,  Yang Yang 2,  Pusen Wang 2,  Weitao Que 2,  Lin Zhong 2,  Zhao Cai 3,  Yang Liu 4,  Liang Yang 5,  Yingxia Liu 6
Affiliations

Affiliations

  • 1 School of Medicine, Southern University of Science and Technology, Shenzhen 518055, People's Republic of China; Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, People's Republic of China.
  • 2 Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, People's Republic of China.
  • 3 Shenzhen Mindray Bio-Medical Electronics Co.,Ltd, Shenzhen 518057, People's Republic of China.
  • 4 Southern University of Science and Technology Hospital, Shenzhen 518055, People's Republic of China.
  • 5 School of Medicine, Southern University of Science and Technology, Shenzhen 518055, People's Republic of China; Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, People's Republic of China. Electronic address: yangl@sustech.edu.cn.
  • 6 Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, People's Republic of China. Electronic address: yingxialiu@hotmail.com.
PMID: 38761489 DOI: 10.1016/j.micres.2024.127750
Abstract

The progress of viral Infection involves numerous transcriptional regulatory events. The identification of the newly synthesized transcripts helps us to understand the replication mechanisms and pathogenesis of the virus. Here, we utilized a time-resolved technique called metabolic RNA labeling approach called thiol(SH)-linked alkylation for the metabolic Sequencing of RNA (SLAM-seq) to differentially elucidate the levels of steady-state and newly synthesized RNAs of BHK21 cell line in response to human coronavirus OC43 (HCoV-OC43) Infection. Our results showed that the Wnt/β-catenin signaling pathway was significantly enriched with the newly synthesized transcripts of BHK21 cell line in response to HCoV-OC43 Infection. Moreover, inhibition of the Wnt pathway promoted viral replication in the early stage of Infection, but inhibited it in the later stage of Infection. Furthermore, remdesivir inhibits the upregulation of the Wnt/β-catenin signaling pathway induced by early Infection with HCoV-OC43. Collectively, our study showed the diverse roles of Wnt/β-catenin pathway at different stages of HCoV-OC43 Infection, suggesting a potential target for the Antiviral treatment. In addition, although Infection with HCoV-OC43 induces cytopathic effects in BHK21 cells, inhibiting Apoptosis does not affect the intracellular replication of the virus. Monitoring newly synthesized RNA based on such time-resolved approach is a highly promising method for studying the mechanism of viral infections.

Keywords

HCoV-OC43; Introduction; Remdesivir; SLAM-seq; Viral infection; Wnt pathway.

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