2. Academic Validation
  3. MiR-221 on protective oxidative induced by selenium modified Codonopsis pilosula polysaccharide

MiR-221 on protective oxidative induced by selenium modified Codonopsis pilosula polysaccharide

  • Int J Biol Macromol. 2024 Aug 21;279(Pt 3):134815. doi: 10.1016/j.ijbiomac.2024.134815.
Tao Qin 1,  Mengke Sun 1,  Yongyuan Huang 2,  Jinhang Guo 1,  Ancan Hong 2,  Qiang Zheng 1,  Tiantian Wei 2,  Qiuyue He 1,  Zhe Ren 3
Affiliations

Affiliations

  • 1 Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • 2 Fujian Key Laboratory of Chinese Traditional and Western Veterinary Medicine and Animal Health, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • 3 Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address: rz1230@163.com.
PMID: 39154690 DOI: 10.1016/j.ijbiomac.2024.134815
Abstract

Oxidative stress plays an important role in various diseases. miR-221 has been reported to regulate oxidative stress. However, the mechanism of miR-221 in regulating oxidative stress induced by sCPPS5 remains unclear. This study aimed to investigate the protective effects and mechanisms of miR-221 on oxidative stress induced by sCPPS5. The expression of SOD, CAT, MDA, LDH, MMP, Caspase-3 activity and Apoptosis were measured. In addition, the key signaling factors in the Keap1-Nrf2-ARE signaling pathway were determined by Real-Time PCR and Western blot. Mice were employed to evaluate the effects of sCPPS5 and the possible mechanism in vivo. sCPPS5 promoted the expression of SOD and CAT and activated Keap1-Nrf2-ARE signaling pathway inhibit the MDA content, MMP, Caspase-3 activity, Apoptosis and LDH release rate after transfection with miR-221 mimics and inhibitors. Consistently, sCPPS5 has the potential to enhance the expression of antioxidant Enzymes as well as upregulate mRNA expression of crucial signal proteins in vivo. miR-221 on oxidative stress protection induced by sCPPS5 possibly through regulating the Keap1-Nrf2-ARE signaling pathway in macrophages.

Keywords

Keap1-Nrf2 signaling pathway; Selenium Codonopsis pilosula polysaccharide; miR-221.

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