2. Academic Validation
  3. MYO3B promotes cancer progression in endometrial cancer by mediating the calcium ion-RhoA/ROCK1 signaling pathway

MYO3B promotes cancer progression in endometrial cancer by mediating the calcium ion-RhoA/ROCK1 signaling pathway

  • J Cancer Res Clin Oncol. 2024 Sep 19;150(9):424. doi: 10.1007/s00432-024-05940-x.
Chunmei Zhang 1,  Huifeng Zhang 2,  Xiaofeng Yang 3,  Sufen Li 1,  Liang Wang 4,  Huancheng Su 1,  Jiaolin Yang 1,  Yuanyuan Ding 1,  Xinglin Zhang 1,  Bao Qiang 1,  Sanyuan Zhang 5
Affiliations

Affiliations

  • 1 Department of Gynecology, First Hospital of Shanxi Medical University, No.85, Jiefang South Road, Yingze District, Taiyuan, Shanxi Province, 030001, China.
  • 2 Department of Basic Medicine, Shanxi Medical University, No.56, Xinjian South Road, Yingze District, Taiyuan, Shanxi Province, 030001, China. vividiny@126.com.
  • 3 Department of Urology, First Hospital of Shanxi Medical University, Taiyuan, 030001, China.
  • 4 Shanxi Inspection and Testing Center, Taiyuan, 030001, China.
  • 5 Department of Gynecology, First Hospital of Shanxi Medical University, No.85, Jiefang South Road, Yingze District, Taiyuan, Shanxi Province, 030001, China. zhl_766880505@outlook.com.
PMID: 39297944 DOI: 10.1007/s00432-024-05940-x
Abstract

Purpose: This study aimed to investigate the effect of MYO3B on endometrial Cancer (EC) proliferation and invasion.

Methods: The expression of MYO3B in EC tissues and cells was analyzed using TCGA database, immunohistochemical staining, Real-Time PCR, and western blot (WB). Cell proliferation was detected by CCK8, Annexin V-APC/PI flow cytometry was used to detect Apoptosis, intracellular calcium ion (CA2+) was detected by flow cytometry with Fluo-4 AM fluorescent probe, cell migration by scratch assay, and cell invasion by Transwell assay, and the expression of proteins related to CA2+ homeostasis and RhoA/ROCK1 signaling pathway was detected by WB and immunofluorescence staining.

Results: The expression of MYO3B was an influential factor in EC recurrence, and the expression of MYO3B was significantly up-regulated in EC tissues and cells, but down-regulated in KLE cells, and MYO3B knockdown inhibited the proliferation, migration, and invasion ability of EC cells and promoted Apoptosis, suggesting that MYO3B plays a tumor-promoting role in EC. Furthermore, MYO3B knockdown decreased CA2+ concentration in EC cells and the RhoA/ROCK1 signaling pathway was inhibited, and the effect of MYO3B knockdown on RhoA/ROCK1 signaling was reversed by treatment with the Calmodulin agonist CALP-2, and the effects of MYO3B knockdown on cell proliferation, migration, and invasion were reversed after treatment with the RhoA agonist U-46,619.

Conclusion: MYO3B promotes the proliferation and migration of endometrial Cancer cells via CA2+-RhoA/ROCK1 signaling pathway. High expression of MYO3B may be a biomarker for EC metastasis.

Keywords

Calcium/calmodulin; Cell proliferation; Endometrial cancer; Myosin 3B; RhoA/ROCK1 signaling.

Figures
Products