2. Academic Validation
  3. Cationized extracellular vesicles for gene delivery

Cationized extracellular vesicles for gene delivery

  • Sci Rep. 2024 Oct 28;14(1):25818. doi: 10.1038/s41598-024-75985-y.
Natalia L Klyachko 1 2,  Matthew J Haney 3 4,  Anton V Lopukhov 5,  Irina M Le-Deygen 5
Affiliations

Affiliations

  • 1 Division of Pharmacoengineering and Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-7362, USA. nlklyachko@gmail.com.
  • 2 Deparment of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia. nlklyachko@gmail.com.
  • 3 Division of Pharmacoengineering and Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-7362, USA.
  • 4 Center for Nanotechnology in Drug Delivery, Carolina Institute for Nanomedicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • 5 Deparment of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
PMID: 39468145 DOI: 10.1038/s41598-024-75985-y
Abstract

Last decade, extracellular vesicles (EVs) attracted a lot of attention as potent versatile drug delivery vehicles. We reported earlier the development of EV-based delivery systems for therapeutic proteins and small molecule chemotherapeutics. In this work, we first time engineered EVs with multivalent Cationic Lipids for the delivery of nucleic acids. Stable, small size cationized EVs were loaded with plasmid DNA (pDNA), or mRNA, or siRNA. Nucleic acid loaded EVs were efficiently taken up by target cells as demonstrated by confocal microscopy and delivered their cargo to the nuclei in triple negative breast Cancer (TNBC) cells and macrophages. Efficient transfection was achieved by engineered cationized EVs formulations of pDNA- and mRNA in vitro. Furthermore, siRNA loaded into cationized EVs showed significant knockdown of the reporter gene in Luc-expressing cells. Overall, multivalent cationized EVs represent a promising strategy for gene delivery.

Keywords

Cancer; EVs; Gene delivery; Multivalent cationic lipid; Transfection.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-144014
    98.34%, Lipid Vector