Hyperosmotic Stress Promotes the Nuclear Translocation of TFEB in Tubular Epithelial Cells Depending on Intracellular Ca2+ Signals via TRPML Channels

Purpose: We previously demonstrated that hyperosmotic stress, which acts as mechanical stress, induces Autophagy of tubular epithelial cells. This study aims to elucidate the molecular mechanisms of hyperosmolarity-induced Autophagy. The research question addresses how hyperosmotic stress activates Autophagy through transcription factor EB (TFEB) and CA²⁺ signaling pathways, contributing to understanding cellular responses to mechanical stress.

Methods: NRK-52E normal rat kidney cells were subjected to hyperosmotic stress using mannitol-containing medium. Fluorescence microscopy was utilized to observe TFEB nuclear translocation, a crucial event in Autophagy regulation. An intracellular CA²⁺ chelator, BAPTA-AM, and a Calcineurin Inhibitor were used to dissect the CA²⁺ signaling pathway involved in TFEB translocation. The phosphorylation of p70S6K, a substrate of the mammalian target of rapamycin complex 1 kinase, was analyzed to explore its role in TFEB localization. Additionally, the function of transient receptor potential mucolipin 1 (TRPML1), an intracellular CA²⁺ channel, was assessed using pharmacological inhibition to determine its impact on TFEB translocation and Autophagy marker LC3-II levels.

Results: Mannitol-induced hyperosmotic stress promoted the nuclear translocation of TFEB, which was completely abolished by treatment with BAPTA-AM. Inhibition of Calcineurin suppressed TFEB nuclear translocation under hyperosmolarity, indicating that a signaling pathway governed by intracellular CA²⁺ is involved in TFEB's nuclear translocation. In contrast, hyperosmotic stress did not significantly alter p70S6K phosphorylation. Pharmacological inhibition of TRPML1 attenuated both TFEB nuclear translocation and LC3-II upregulation in response to hyperosmotic stress.

Conclusions: Hyperosmotic stress promotes TFEB nuclear localization, and TRPML1-induced activation of Calcineurin is involved in the mechanism of hyperosmolarity-induced Autophagy.

Supplementary information: The online version contains supplementary material available at 10.1007/s12195-024-00839-6.

Calcineurin; Hyperosmolarity; TRPML1; Transcription factor EB (TFEB); Tubular epithelial cell.