2. Epigenetics PI3K/Akt/mTOR Autophagy
  3. AMPK Autophagy Mitophagy
  4. Metformin-d6 hydrochloride

Metformin-d6 hydrochloride  (Synonyms: 1,1-Dimethylbiguanide-d6 hydrochloride)

Cat. No.: HY-110228 Purity: 98.79%
COA Handling Instructions

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Metformin-d6 (hydrochloride)e is a deuterium labeled Metformin hydrochloride. Metformin hydrochloride inhibits the mitochondrial respiratory chain in the liver, leading to activation of AMPK, enhancing insulin sensitivity for type 2 diabetes research. Metformin hydrochloride triggers autophagy[1].

For research use only. We do not sell to patients.

Metformin-d<sub>6</sub> hydrochloride Chemical Structure

Metformin-d6 hydrochloride Chemical Structure

CAS No. : 1185166-01-1

Size Price Stock Quantity
1 mg USD 115 In-stock
5 mg USD 400 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Metformin-d6 hydrochloride:

Metformin-d6 hydrochloride Related Antibodies

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

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  • Purity & Documentation

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Description

Metformin-d6 (hydrochloride)e is a deuterium labeled Metformin hydrochloride. Metformin hydrochloride inhibits the mitochondrial respiratory chain in the liver, leading to activation of AMPK, enhancing insulin sensitivity for type 2 diabetes research. Metformin hydrochloride triggers autophagy[1].

IC50 & Target[1]

AMPK

 

Molecular Weight

171.66

Formula

C4H6D6ClN5
CAS No.
Unlabeled CAS

1115-70-4
Appearance

Solid

Color

White to off-white

SMILES

NC(NC(N(C([2H])([2H])[2H])C([2H])([2H])[2H])=N)=N.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (291.27 mM; Need ultrasonic)

DMSO : 50 mg/mL (291.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.8255 mL 29.1273 mL 58.2547 mL
5 mM 1.1651 mL 5.8255 mL 11.6509 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (12.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (12.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.79%

COA (249 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.8255 mL 29.1273 mL 58.2547 mL 145.6367 mL
5 mM 1.1651 mL 5.8255 mL 11.6509 mL 29.1273 mL
10 mM 0.5825 mL 2.9127 mL 5.8255 mL 14.5637 mL
15 mM 0.3884 mL 1.9418 mL 3.8836 mL 9.7091 mL
20 mM 0.2913 mL 1.4564 mL 2.9127 mL 7.2818 mL
25 mM 0.2330 mL 1.1651 mL 2.3302 mL 5.8255 mL
30 mM 0.1942 mL 0.9709 mL 1.9418 mL 4.8546 mL
40 mM 0.1456 mL 0.7282 mL 1.4564 mL 3.6409 mL
50 mM 0.1165 mL 0.5825 mL 1.1651 mL 2.9127 mL
60 mM 0.0971 mL 0.4855 mL 0.9709 mL 2.4273 mL
80 mM 0.0728 mL 0.3641 mL 0.7282 mL 1.8205 mL
100 mM 0.0583 mL 0.2913 mL 0.5825 mL 1.4564 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Metformin-d6 hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Metformin-d61185166-01-11,1-Dimethylbiguanide-d6AMPKAutophagyMitophagyAMP-activated protein kinaseMitochondrial AutophagyInhibitorinhibitorinhibit

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