Methotrexate α-tert-butyl ester

Name: Methotrexate α-tert-butyl ester
Brand: MedChemExpress
SKU: HY-133887
Rating: 4.5 (1 reviews)

Cat. No.: HY-133887 Purity: ≥96.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Methotrexate α-tert-butyl ester, capped by OtBu, significantly reduces tumor growth in HT1080 tumor bearing mice. Methotrexate is an antimetabolite and antifolate agent and is also an immunosuppressant and antineoplastic agent.

For research use only. We do not sell to patients.

Methotrexate α-tert-butyl ester Chemical Structure

CAS No. : 79640-70-3

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
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100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

OtBu-MTX-PVG is less HT1080 than MTX-PVG against HT1080 cells. It shows that protection of the α-carboxyl group of MTX results in reduced in vitro activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Lys-MTXcleavable- (OtBu) significantly reduces tumor growth in HT1080 tumor bearing mice^[1].
The protection of the α-carboxyl group of methotrexate may be used to improve the circulatory half-life and reduce the liver accumulation of similar MTX-conjugated dendrimers, while still retaining antitumor activity in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female, Balb/c nu/nu, 6 weeks (bearing HT1080 cells)^[1]
Dosage:	30 mg/kg
Administration:	Once per week for 2 weeks
Result:	Showed a significant (63%) reduction in tumor size on day 12.

Molecular Weight

510.55

Formula

C₂₄H₃₀N₈O₅

CAS No.

79640-70-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)CC[C@H](NC(C1=CC=C(N(CC2=NC3=C(N)N=C(N)N=C3N=C2)C)C=C1)=O)C(OC(C)(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (195.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9587 mL	9.7934 mL	19.5867 mL
5 mM	0.3917 mL	1.9587 mL	3.9173 mL
10 mM	0.1959 mL	0.9793 mL	1.9587 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥96.0%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (250 KB) Elemental Analysis Report (108 KB)

Handling Instructions (2659 KB)

References

[1]. Kaminskas LM, et al. Capping methotrexate α-carboxyl groups enhances systemic exposure and retains the cytotoxicity of drug conjugated PEGylated polylysine dendrimers. Mol Pharm. 2011;8(2):338-349. [Content Brief]

[2]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73. [Content Brief]

[3]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92. [Content Brief]

[4]. Ehab Tousson, et al. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9587 mL	9.7934 mL	19.5867 mL	48.9668 mL
	5 mM	0.3917 mL	1.9587 mL	3.9173 mL	9.7934 mL
	10 mM	0.1959 mL	0.9793 mL	1.9587 mL	4.8967 mL
	15 mM	0.1306 mL	0.6529 mL	1.3058 mL	3.2645 mL
	20 mM	0.0979 mL	0.4897 mL	0.9793 mL	2.4483 mL
	25 mM	0.0783 mL	0.3917 mL	0.7835 mL	1.9587 mL
	30 mM	0.0653 mL	0.3264 mL	0.6529 mL	1.6322 mL
	40 mM	0.0490 mL	0.2448 mL	0.4897 mL	1.2242 mL
	50 mM	0.0392 mL	0.1959 mL	0.3917 mL	0.9793 mL
	60 mM	0.0326 mL	0.1632 mL	0.3264 mL	0.8161 mL
	80 mM	0.0245 mL	0.1224 mL	0.2448 mL	0.6121 mL
	100 mM	0.0196 mL	0.0979 mL	0.1959 mL	0.4897 mL