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  4. N-(9-Acridinyl)maleimide

N-(9-Acridinyl)maleimide is a maleimide type fluorescent thiol reagent. N-(9-Acridinyl)maleimide shows no substantial fluorescence but its coupling products with thiol compounds exhibit strong blue fluorescence. N-(9-Acridinyl)maleimide is used for fluorometrical analysis of cysteine and glutathione.

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N-(9-Acridinyl)maleimide Chemical Structure

N-(9-Acridinyl)maleimide Chemical Structure

CAS No. : 49759-20-8

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5 mg USD 34 In-stock
10 mg USD 55 In-stock
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N-(9-Acridinyl)maleimide Related Antibodies

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Description

N-(9-Acridinyl)maleimide is a maleimide type fluorescent thiol reagent. N-(9-Acridinyl)maleimide shows no substantial fluorescence but its coupling products with thiol compounds exhibit strong blue fluorescence. N-(9-Acridinyl)maleimide is used for fluorometrical analysis of cysteine and glutathione[1].

Molecular Weight

274.28

Formula

C17H10N2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

454
Excitation (Ex)

344
SMILES

O=C(N1C2=C(C=CC=C3)C3=NC4=CC=CC=C42)C=CC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (18.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6459 mL 18.2295 mL 36.4591 mL
5 mM 0.7292 mL 3.6459 mL 7.2918 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6459 mL 18.2295 mL 36.4591 mL 91.1477 mL
5 mM 0.7292 mL 3.6459 mL 7.2918 mL 18.2295 mL
10 mM 0.3646 mL 1.8230 mL 3.6459 mL 9.1148 mL
15 mM 0.2431 mL 1.2153 mL 2.4306 mL 6.0765 mL
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N-(9-Acridinyl)maleimide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-(9-Acridinyl)maleimide49759-20-8Fluorescent DyeMaleimidefluorescentthiolbluefluorometricalcysteineglutathioneInhibitorinhibitorinhibit

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