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  4. N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide  (Synonyms: Ac-IEPD-pNA)

Cat. No.: HY-P3236 Purity: 98.52%
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N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a granzyme B substrate that allows accurate measurement of granzyme B activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Chemical Structure

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Chemical Structure

CAS No. : 216757-29-8

Size Price Stock Quantity
5 mg USD 126 In-stock
10 mg USD 202 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Related Antibodies

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Description

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a granzyme B substrate that allows accurate measurement of granzyme B activity[1].

Molecular Weight

634.63

Formula

C28H38N6O11
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ac-Ile-Glu-Pro-Asp-{pNA}
Sequence Shortening

Ac-IEPD-{pNA}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (157.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5757 mL 7.8786 mL 15.7572 mL
5 mM 0.3151 mL 1.5757 mL 3.1514 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5757 mL 7.8786 mL 15.7572 mL 39.3930 mL
5 mM 0.3151 mL 1.5757 mL 3.1514 mL 7.8786 mL
10 mM 0.1576 mL 0.7879 mL 1.5757 mL 3.9393 mL
15 mM 0.1050 mL 0.5252 mL 1.0505 mL 2.6262 mL
20 mM 0.0788 mL 0.3939 mL 0.7879 mL 1.9697 mL
25 mM 0.0630 mL 0.3151 mL 0.6303 mL 1.5757 mL
30 mM 0.0525 mL 0.2626 mL 0.5252 mL 1.3131 mL
40 mM 0.0394 mL 0.1970 mL 0.3939 mL 0.9848 mL
50 mM 0.0315 mL 0.1576 mL 0.3151 mL 0.7879 mL
60 mM 0.0263 mL 0.1313 mL 0.2626 mL 0.6566 mL
80 mM 0.0197 mL 0.0985 mL 0.1970 mL 0.4924 mL
100 mM 0.0158 mL 0.0788 mL 0.1576 mL 0.3939 mL
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N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide216757-29-8Ac-IEPD-pNAFluorescent DyeInhibitorinhibitorinhibit

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