N-(Benzyloxycarbonyloxy)succinimide

Name: N-(Benzyloxycarbonyloxy)succinimide
Brand: MedChemExpress
SKU: HY-75200
Rating: 4.5 (1 reviews)

Cat. No.: HY-75200 Purity: 98.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N-(Benzyloxycarbonyloxy)succinimide is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

N-(Benzyloxycarbonyloxy)succinimide Chemical Structure

CAS No. : 13139-17-8

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Purity & Documentation
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Description

N-(Benzyloxycarbonyloxy)succinimide is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Molecular Weight

249.22

Formula

C₁₂H₁₁NO₅

CAS No.

13139-17-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCC1=CC=CC=C1)ON2C(CCC2=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (401.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0125 mL	20.0626 mL	40.1252 mL
5 mM	0.8025 mL	4.0125 mL	8.0250 mL
10 mM	0.4013 mL	2.0063 mL	4.0125 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (244 KB) SDS (393 KB)

COA (250 KB) HNMR (153 KB) RP-HPLC (226 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0125 mL	20.0626 mL	40.1252 mL	100.3130 mL
	5 mM	0.8025 mL	4.0125 mL	8.0250 mL	20.0626 mL
	10 mM	0.4013 mL	2.0063 mL	4.0125 mL	10.0313 mL
	15 mM	0.2675 mL	1.3375 mL	2.6750 mL	6.6875 mL
	20 mM	0.2006 mL	1.0031 mL	2.0063 mL	5.0156 mL
	25 mM	0.1605 mL	0.8025 mL	1.6050 mL	4.0125 mL
	30 mM	0.1338 mL	0.6688 mL	1.3375 mL	3.3438 mL
	40 mM	0.1003 mL	0.5016 mL	1.0031 mL	2.5078 mL
	50 mM	0.0803 mL	0.4013 mL	0.8025 mL	2.0063 mL
	60 mM	0.0669 mL	0.3344 mL	0.6688 mL	1.6719 mL
	80 mM	0.0502 mL	0.2508 mL	0.5016 mL	1.2539 mL
	100 mM	0.0401 mL	0.2006 mL	0.4013 mL	1.0031 mL