2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. Cathepsin Deubiquitinase
  4. N-Ethylmaleimide-d5

N-Ethylmaleimide-d5  (Synonyms: NEM-d5)

Cat. No.: HY-D0843S Purity: 99.49%
COA Handling Instructions

Molarity Calculator

N-Ethylmaleimide-d5 is the deuterium labeled N-Ethylmaleimide. N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[1]. N-ethylmaleimide specific inhibits phosphate transport in mitochondria[2]. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor[3].

For research use only. We do not sell to patients.

N-Ethylmaleimide-d<sub>5</sub> Chemical Structure

N-Ethylmaleimide-d5 Chemical Structure

CAS No. : 360768-37-2

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1 mg USD 190 In-stock
5 mg USD 535 In-stock
10 mg USD 740 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of N-Ethylmaleimide-d5:

N-Ethylmaleimide-d5 Related Antibodies

Top Publications Citing Use of Products

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Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

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USP1 USP2 USP7 USP8 USP30 UCHL1

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  • Purity & Documentation

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Description

N-Ethylmaleimide-d5 is the deuterium labeled N-Ethylmaleimide. N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[1]. N-ethylmaleimide specific inhibits phosphate transport in mitochondria[2]. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor[3].

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N-Ethylmaleimide-d5 Related Antibodies
Molecular Weight

130.16

Formula

C6H2D5NO2
CAS No.
Unlabeled CAS

128-53-0
Appearance

Solid

Color

White to off-white

SMILES

O=C(C=C1)N(C([2H])([2H])C([2H])([2H])[2H])C1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (768.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (384.14 mM; Need ultrasonic)

H2O : 50 mg/mL (384.14 mM; Need ultrasonic)

Ethanol : 12.5 mg/mL (96.04 mM; Need ultrasonic)

Ethanol : 12.5 mg/mL (96.04 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.6829 mL 38.4143 mL 76.8285 mL
5 mM 1.5366 mL 7.6829 mL 15.3657 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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g

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.49%

COA (260 KB) HNMR (157 KB) RP-HPLC (236 KB) LCMS (103 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / Ethanol / H2O / H2O / DMSO 1 mM 7.6829 mL 38.4143 mL 76.8285 mL 192.0713 mL
5 mM 1.5366 mL 7.6829 mL 15.3657 mL 38.4143 mL
10 mM 0.7683 mL 3.8414 mL 7.6829 mL 19.2071 mL
15 mM 0.5122 mL 2.5610 mL 5.1219 mL 12.8048 mL
20 mM 0.3841 mL 1.9207 mL 3.8414 mL 9.6036 mL
25 mM 0.3073 mL 1.5366 mL 3.0731 mL 7.6829 mL
30 mM 0.2561 mL 1.2805 mL 2.5610 mL 6.4024 mL
40 mM 0.1921 mL 0.9604 mL 1.9207 mL 4.8018 mL
50 mM 0.1537 mL 0.7683 mL 1.5366 mL 3.8414 mL
60 mM 0.1280 mL 0.6402 mL 1.2805 mL 3.2012 mL
80 mM 0.0960 mL 0.4802 mL 0.9604 mL 2.4009 mL
H2O / H2O / DMSO 100 mM 0.0768 mL 0.3841 mL 0.7683 mL 1.9207 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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N-Ethylmaleimide-d5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Ethylmaleimide-d5360768-37-2NEM-d5CathepsinDeubiquitinaseDUBsInhibitorinhibitorinhibit

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