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  4. N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate

N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate  (Synonyms: TCFH)

Cat. No.: HY-W011556 Purity: ≥99.0%
COA Handling Instructions

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N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate is a kind of biological coupling reagent, can be used for antitumor drugs sulphur alkali before medicine research.

For research use only. We do not sell to patients.

N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate Chemical Structure

N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate Chemical Structure

CAS No. : 94790-35-9

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Based on 1 publication(s) in Google Scholar

N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate is a kind of biological coupling reagent, can be used for antitumor drugs sulphur alkali before medicine research[1].

Molecular Weight

280.58

Formula

C5H12ClF6N2P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C/[N+](C)=C(Cl)\N(C)C.F[P-](F)(F)(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (356.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5640 mL 17.8202 mL 35.6405 mL
5 mM 0.7128 mL 3.5640 mL 7.1281 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

COA (243 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5640 mL 17.8202 mL 35.6405 mL 89.1012 mL
5 mM 0.7128 mL 3.5640 mL 7.1281 mL 17.8202 mL
10 mM 0.3564 mL 1.7820 mL 3.5640 mL 8.9101 mL
15 mM 0.2376 mL 1.1880 mL 2.3760 mL 5.9401 mL
20 mM 0.1782 mL 0.8910 mL 1.7820 mL 4.4551 mL
25 mM 0.1426 mL 0.7128 mL 1.4256 mL 3.5640 mL
30 mM 0.1188 mL 0.5940 mL 1.1880 mL 2.9700 mL
40 mM 0.0891 mL 0.4455 mL 0.8910 mL 2.2275 mL
50 mM 0.0713 mL 0.3564 mL 0.7128 mL 1.7820 mL
60 mM 0.0594 mL 0.2970 mL 0.5940 mL 1.4850 mL
80 mM 0.0446 mL 0.2228 mL 0.4455 mL 1.1138 mL
100 mM 0.0356 mL 0.1782 mL 0.3564 mL 0.8910 mL
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N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N,N,N',N'-Tetramethylchloroformamidinium94790-35-9TCFHBiochemical Assay Reagentsbiochemical reagentbiological materialorganic compoundlife scienceInhibitorinhibitorinhibit

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