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Naph-EA-mal  (Synonyms: Thiol-green 1)

Cat. No.: HY-D1261 Purity: ≥95.0%
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Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.

For research use only. We do not sell to patients.

Naph-EA-mal Chemical Structure

Naph-EA-mal Chemical Structure

CAS No. : 210292-65-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 330 In-stock
Solution
10 mM * 1 mL in DMSO USD 330 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
50 mg   Get quote  
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Based on 1 publication(s) in Google Scholar

Naph-EA-mal Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Naph-EA-mal

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Description

Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells[1]. Ex: 488 nM; Em: 540 nM.

In Vitro

Imaging thiols in living cells[1]
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells[1].
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed[1].
The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again[1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Naph-EA-mal Related Antibodies
Molecular Weight

391.42

Formula

C22H21N3O4
CAS No.
Appearance

Solid

Color

Light yellow to orange

Emission (Em)

540
Excitation (Ex)

488
SMILES

O=C1N(CCCC)C(C2=CC=C(NCCN3C(C=CC3=O)=O)C4=CC=CC1=C24)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (85.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5548 mL 12.7740 mL 25.5480 mL
5 mM 0.5110 mL 2.5548 mL 5.1096 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
Dyeing Example
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5548 mL 12.7740 mL 25.5480 mL 63.8700 mL
5 mM 0.5110 mL 2.5548 mL 5.1096 mL 12.7740 mL
10 mM 0.2555 mL 1.2774 mL 2.5548 mL 6.3870 mL
15 mM 0.1703 mL 0.8516 mL 1.7032 mL 4.2580 mL
20 mM 0.1277 mL 0.6387 mL 1.2774 mL 3.1935 mL
25 mM 0.1022 mL 0.5110 mL 1.0219 mL 2.5548 mL
30 mM 0.0852 mL 0.4258 mL 0.8516 mL 2.1290 mL
40 mM 0.0639 mL 0.3194 mL 0.6387 mL 1.5968 mL
50 mM 0.0511 mL 0.2555 mL 0.5110 mL 1.2774 mL
60 mM 0.0426 mL 0.2129 mL 0.4258 mL 1.0645 mL
80 mM 0.0319 mL 0.1597 mL 0.3194 mL 0.7984 mL
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Naph-EA-mal Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naph-EA-mal210292-65-2Thiol-green 1Fluorescent DyeNEMthiol-disulfidethiolintracellularInhibitorinhibitorinhibit

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