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  3. Naphthol AS-BI phosphate

Naphthol AS-BI phosphate is a substrate for acid and alkaline phosphatase and can be used in fluorescence assays. Naphthol AS-BI phosphate is a kind of biological materials or organic compounds that are widely used in life science research.

For research use only. We do not sell to patients.

Naphthol AS-BI phosphate Chemical Structure

Naphthol AS-BI phosphate Chemical Structure

CAS No. : 1919-91-1

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Based on 1 publication(s) in Google Scholar

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Description

Naphthol AS-BI phosphate is a substrate for acid and alkaline phosphatase and can be used in fluorescence assays. Naphthol AS-BI phosphate is a kind of biological materials or organic compounds that are widely used in life science research[1].

Molecular Weight

452.19

Formula

C18H15BrNO6P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C(OP(O)(O)=O)C=C2C=CC(Br)=CC2=C1)NC3=C(OC)C=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 175 mg/mL (387.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2115 mL 11.0573 mL 22.1146 mL
5 mM 0.4423 mL 2.2115 mL 4.4229 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2115 mL 11.0573 mL 22.1146 mL 55.2865 mL
5 mM 0.4423 mL 2.2115 mL 4.4229 mL 11.0573 mL
10 mM 0.2211 mL 1.1057 mL 2.2115 mL 5.5286 mL
15 mM 0.1474 mL 0.7372 mL 1.4743 mL 3.6858 mL
20 mM 0.1106 mL 0.5529 mL 1.1057 mL 2.7643 mL
25 mM 0.0885 mL 0.4423 mL 0.8846 mL 2.2115 mL
30 mM 0.0737 mL 0.3686 mL 0.7372 mL 1.8429 mL
40 mM 0.0553 mL 0.2764 mL 0.5529 mL 1.3822 mL
50 mM 0.0442 mL 0.2211 mL 0.4423 mL 1.1057 mL
60 mM 0.0369 mL 0.1843 mL 0.3686 mL 0.9214 mL
80 mM 0.0276 mL 0.1382 mL 0.2764 mL 0.6911 mL
100 mM 0.0221 mL 0.1106 mL 0.2211 mL 0.5529 mL
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Naphthol AS-BI phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Naphthol AS-BI phosphate
Cat. No.:
HY-131475
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