2. Apoptosis
  3. Necroptosis RIP kinase
  4. Necrostatin-5

Necrostatin-5  (Synonyms: Nec-5)

Cat. No.: HY-124546 Purity: 98.43%
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Necrostatin-5 (Nec-5) is a potent necroptosis inhibitor with an EC50 value of 0.24 µM. Necrostatin-5 also is a RIP1 inhibitor. Necrostatin-5 shows cardioprotective effects.

For research use only. We do not sell to patients.

Necrostatin-5 Chemical Structure

Necrostatin-5 Chemical Structure

CAS No. : 337349-54-9

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Based on 1 publication(s) in Google Scholar

Necrostatin-5 Related Antibodies

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Description

Necrostatin-5 (Nec-5) is a potent necroptosis inhibitor with an EC50 value of 0.24 µM. Necrostatin-5 also is a RIP1 inhibitor. Necrostatin-5 shows cardioprotective effects[1][2][3].

IC50 & Target[1][3]

RIPK1

 

necroptosis

0.24 μM (EC50)

In Vitro

Necrostatin-5 (1, 5, 10, 50, 100 µM) inhibits LDH release in a dose-dependent mnner[3].
Necrostatin-5 (100 µM) protects MH-S cells against challenge with S. aureus, Listeria monocytogenes, S. pneumoniae, uropathogenic Escherichia coli (UPEC), and protectes against pneumolysin-induced death[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Necrostatin-5 Related Antibodies
In Vivo

Necrostatin-5 (2.46 mg/kg; i.p.) shows cardioprotective effects on the isolated heart model in rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 200-300 g, male Wistar rats[2]
Dosage: 2.46 mg/kg
Administration: I.p.; 60 min before experiment
Result: Reduced the infarction zone caused by 30-min global ischemia and 120-min reperfusion.
Molecular Weight

383.49

Formula

C19H17N3O2S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C2=CC=C(C=C2)OC)C3=C(N=C1SCC#N)SC4=C3CCCC4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (13.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6076 mL 13.0381 mL 26.0763 mL
5 mM 0.5215 mL 2.6076 mL 5.2153 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6076 mL 13.0381 mL 26.0763 mL 65.1907 mL
5 mM 0.5215 mL 2.6076 mL 5.2153 mL 13.0381 mL
10 mM 0.2608 mL 1.3038 mL 2.6076 mL 6.5191 mL
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Necrostatin-5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Necrostatin-5337349-54-9Nec-5Necrostatin5Necrostatin 5Nec5Nec 5NecroptosisRIP kinaseReceptor-interacting protein kinasesRIPKLDHcardioprotectiveMH-S cellsInhibitorinhibitorinhibit

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