2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. Nemadipine-A

Nemadipine-A is a specific inhibitor of the EGL-19 L-type Ca2+ channel. Nemadipine-A, a cell-permeable L-type calcium channel inhibitor, sensitizes TRAIL-resistant cancer cells to this ligand.

For research use only. We do not sell to patients.

Nemadipine-A Chemical Structure

Nemadipine-A Chemical Structure

CAS No. : 54280-71-6

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Based on 1 publication(s) in Google Scholar

Nemadipine-A Related Antibodies

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Description

Nemadipine-A is a specific inhibitor of the EGL-19 L-type Ca2+ channel[1]. Nemadipine-A, a cell-permeable L-type calcium channel inhibitor, sensitizes TRAIL-resistant cancer cells to this ligand[2].

In Vitro

Nemadipine-A is a specific and highly effective inhibitor of EGL-19. Nemadipine-A (µM) is sufficient to abolish the enhanced level of paralysis observed in egl-19(ad695); crIs4 mutants[1].
Nemadipine-A strongly potentiates TRAIL-induced apoptosis in TRAIL-resistant lung cancer cells via the down-regulation of the anti-apoptotic protein survivin[2]. Nemadipine-A sensitizes H1299 lung cancer cells towards TRAIL-induced cytotoxicity[2]. Combination of Nemadipine-A (20 μM) with TRAIL (20 ng/mL) induces caspase-mediated apoptotic cell death[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nemadipine-A Related Antibodies

Cell Viability Assay[2]

Cell Line: H1299 lung adenocarcinoma cells
Concentration: 0, 5, 10, 20, and 30 μM
Incubation Time: 8 hours
Result: H1299 cells were resistant to the apoptotic effects of TRAIL, but the co-administration of Nemadipine-A and TRAIL in these cells resulted in notably increased cytotoxicity in a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: H1299 cells
Concentration: 20 μM
Incubation Time: 8 hours
Result: Co-treated with TRAIL and Nemadipine-A (T/N; 20 ng/mL and 20 μM respectively) induced cell death in H1299 cells.
Molecular Weight

419.34

Formula

C19H18F5NO4
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CC1=C(C(C(C(OCC)=O)=C(N1)C)C2=C(C(F)=C(C(F)=C2F)F)F)C(OCC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (47.69 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3847 mL 11.9235 mL 23.8470 mL
5 mM 0.4769 mL 2.3847 mL 4.7694 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 98.0%

SDS (393 KB)

COA (251 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3847 mL 11.9235 mL 23.8470 mL 59.6175 mL
5 mM 0.4769 mL 2.3847 mL 4.7694 mL 11.9235 mL
10 mM 0.2385 mL 1.1923 mL 2.3847 mL 5.9617 mL
15 mM 0.1590 mL 0.7949 mL 1.5898 mL 3.9745 mL
20 mM 0.1192 mL 0.5962 mL 1.1923 mL 2.9809 mL
25 mM 0.0954 mL 0.4769 mL 0.9539 mL 2.3847 mL
30 mM 0.0795 mL 0.3974 mL 0.7949 mL 1.9872 mL
40 mM 0.0596 mL 0.2981 mL 0.5962 mL 1.4904 mL
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Nemadipine-A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nemadipine-A54280-71-6Calcium ChannelCa2+ channelsCa channelsEGL-19L-typeCa2+channelcalciumTRAILresistantcancerSensitizationH1299Inhibitorinhibitorinhibit

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