2. Membrane Transporter/Ion Channel
  3. Potassium Channel
  4. NS3623

NS3623 is an activator of human ether-a-go-go-related gene (hERG1/KV11.1) potassium channels. NS3623 activates the IKr and Ito currents and has antiarrhythmic effect. NS3623 has a dual mode of action, being an inhibitor of hERG1 channels.

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NS3623 Chemical Structure

NS3623 Chemical Structure

CAS No. : 343630-41-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 110 In-stock
Solution
10 mM * 1 mL in DMSO USD 110 In-stock
Solid
5 mg USD 100 In-stock
10 mg USD 160 In-stock
25 mg USD 320 In-stock
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Based on 1 publication(s) in Google Scholar

NS3623 Related Antibodies

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Description

NS3623 is an activator of human ether-a-go-go-related gene (hERG1/KV11.1) potassium channels. NS3623 activates the IKr and Ito currents and has antiarrhythmic effect. NS3623 has a dual mode of action, being an inhibitor of hERG1 channels[1][2][3].

In Vitro

In isolated canine cardiomyocytes, application of NS3623 (5 μM) greatly increases the size of the tail current confirming previous studies demonstrating this drug increased IKr. The effect of NS3623 on IKr recorded from cells kept in culture is evaluated. After 48 h, the magnitude of IKr is greatly reduced compared to freshly isolated cells. Peak IKr tail currents are 0.47 ± 0.08 pA/pF in freshly isolated myocytes and 0.28 ± 0.06 pA/pF in 2 day cultured myocytes. Application of NS3623 increases IKr in cultured Mid cells. Analysis of the current-voltage (I-V) relation of IKr tail current showed that NS3623 increased current density in both 1 day and 2 day cultured Mid cells. On a percentage basis, NS3623 increases IKr tail currents (at +50 mV activating pulse) by 60% in normal Mid cells, and 68% in 48 h cultured cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NS3623 Related Antibodies
In Vivo

NS3623 (30 mg/kg; i.v.; lasting for 3 minutes) shortens the corrected QT interval by 25 +/- 4% in anaesthetized guinea pigs[1].
NS3623 (50 mg/kg) shortenes the QT interval by 30 +/- 6% in conscious guinea pigs. NS3623 (50 mg/kg) immediately decreases the QTcF interval and remains significantly shortened for approximately 30 minutes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Anesthetized guinea pig[1]
Dosage: 30 mg/kg
Administration: Intravenous injection; lasting for 3 minutes
Result: Shortened the corrected QT interval by 25 +/- 4%.
Molecular Weight

427.18

Formula

C15H10BrF3N6O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=CC(C(F)(F)F)=C1)NC2=CC=C(Br)C=C2C3=NNN=N3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (585.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3409 mL 11.7047 mL 23.4093 mL
5 mM 0.4682 mL 2.3409 mL 4.6819 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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+
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

SDS (393 KB)

COA (247 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3409 mL 11.7047 mL 23.4093 mL 58.5233 mL
5 mM 0.4682 mL 2.3409 mL 4.6819 mL 11.7047 mL
10 mM 0.2341 mL 1.1705 mL 2.3409 mL 5.8523 mL
15 mM 0.1561 mL 0.7803 mL 1.5606 mL 3.9016 mL
20 mM 0.1170 mL 0.5852 mL 1.1705 mL 2.9262 mL
25 mM 0.0936 mL 0.4682 mL 0.9364 mL 2.3409 mL
30 mM 0.0780 mL 0.3902 mL 0.7803 mL 1.9508 mL
40 mM 0.0585 mL 0.2926 mL 0.5852 mL 1.4631 mL
50 mM 0.0468 mL 0.2341 mL 0.4682 mL 1.1705 mL
60 mM 0.0390 mL 0.1951 mL 0.3902 mL 0.9754 mL
80 mM 0.0293 mL 0.1463 mL 0.2926 mL 0.7315 mL
100 mM 0.0234 mL 0.1170 mL 0.2341 mL 0.5852 mL
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NS3623 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NS3623343630-41-1NS 3623NS-3623Potassium ChannelKcsAPotassiumionchannelscurrentQTAntiarrhythmiceffectcaninecardiomyocytesmidmyocardialcellsInhibitorinhibitorinhibit

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